February

- Our team was formally founded.
- All student members learned the fundamental knowledge of molecular cloning and iGEM.
- All student members looked through the previous excellent projects and learned the basic notion of synthetic biology and iGEM.
-We decided our project theme.

Mars、April、May

- We were divided into three groups and polish our project separately (and we reported each’s progress every weekend).
- Our team was formally accepted by iGEM.

June

-All student members came back to school and learned laboratory fundamental qualities and received molecular cloning operation training.

7.13

-BBa_E0030 and BBa_E0420 are got from 2019 Spring Kit.

7.16

-BBa_K333045is constructed.

7.17

-BBa_K3332046 and BBa_K3332051 are constructed.

7.18

-BBa_K3332056 is constructed

7.21

-BBa_K3332057is constructed and identified by PCR .

7.24

-BBa_K3332061 is constructed successfully to verify INPNC's function and identified by PCR

7.26

-BBa_K3332065 and BBa_K3332066 are constructed to get J23100-RBS-phnE1 and J23100-RBS-phnE2

7.27

-BBa_K3332068 is constructed successfully.

7.30

-BBa_K3332065 and BBa_K3332066 are successfully ligated together to construct BBa_K3332067

8.1

-BBa_K3332069 is successfully constructed by point mutation

8.6

-BBa_K3332072 is constructed to get J23100-RBS-phnO-His-Tag

8.9

-BBa_K3332052 is constructed

8.10

-BBa_K3332047 is constructed successfully.

8.12

-BBa_K3332049 is constructed.

8.14

-BBa_K3332073,BBa_K3332075,BBa_K3332076 andBBa_K3332077 are constructed for RNAi

8.16

-BBa_K3332038 is constructed successfully by PCR

8.20

-BBa_K3332063 is constructed to verify AIDA's function and identified by PCR.

8.25

-BBa_K3332040 is constructed.

9.3

BBa_K3332059 and BBa_K3332060are constructed successfully to anchor GRHPR to the membrane of E. Coli.

-BBa_K2922039 (3195 bp) successfully constructed.

9.13

BBa_I0500 is constructed

9.15

BBa_K3332080 and BBa_K3332081is constructed by ligating BBa_I0500, BBa_Q04510 BBa_K1096002.

9.18

BBa_K3332091 and BBa_K3332092are constructed as an improvement of BBa_K1334002 .

9.19

BBa_K3332093 is constructed as an improvement of BBa_K1334002.

9.20

BBa_K3332084 is constructed by ligating BBa_K3332034andBBa_E0420.

9.23

BBa_K3332086 is constructed.

9.25

BBa_K3332087 is constructed by ligating BBa_K3332040 and BBa_E0420.

9.28

BBa_K3332082 is constructed by ligating BBa_E0430.

BBa_K3332064 is constructed to verify BrkA 's function, which is identified by PCR

10.7

BBa_K3332054 and BBa_K3332055are successfully constructed

10.8

BBa_K3332090is constructed to characterize the function of the improved promoter.

10.10

BBa_K3332097 is constructed by ligatingBBa_K3332068are successfully constructed

10.11

BBa_K3332099 is constructed

10.15

BBa_K3332097 and BBa_K3332068are constructed