Difference between revisions of "Team:TU Darmstadt/Project/Backbones"

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         </div>
 
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<div class="container-noborders">
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        <div class="table-responsive-sm">
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          <table class="table table-light">
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            <tr>
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              <th scope="col">Name</th>
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              <th scope="col">Resistance</th>
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              <th scope="col">Size</th>
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              <th scope="col">Features</th>
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              <th scope="col">Used for the following parts</th>
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            </tr>
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            <tr>
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              <td>pACYCT2</td>
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              <td>Cmp</td>
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              <td>3975 bp</td>
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              <td>
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                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7
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                promoter, lac operator, T7 terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000 "
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                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187001 "
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                    target="_blank"> BBa_K3187001</a>, <a href=" http://parts.igem.org/Part:BBa_K3187003"
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                    target="_blank"> BBa_K3187003</a>, <a href=" http://parts.igem.org/Part:BBa_K3187004" target="_blank"> BBa_K3187004</a>
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</td>
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            </tr>
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            <tr></tr>
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            <tr>
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              <td>pDESTara2</td>
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              <td>Amp</td>
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              <td>4556 bp</td>
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              <td>AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator
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                promoter, lac operator, T7 terminator</td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187010 "
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                    target="_blank"> BBa_K3187010</a>
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</td>
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            </tr>
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            <tr>
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              <td>pET24</td>
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              <td>Kan</td>
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              <td>5236 bp</td>
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              <td>
 +
                KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI
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                repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7
 +
                terminator
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              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187000"
 +
                    target="_blank"> BBa_K3187000</a>, <a href=" http://parts.igem.org/Part:BBa_K3187002 "
 +
                    target="_blank"> BBa_K3187002</a>, <a href=" http://parts.igem.org/Part:BBa_K3187005 "
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                    target="_blank"> BBa_K3187005</a>, <a href=" http://parts.igem.org/Part:BBa_K3187006 "
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                    target="_blank"> BBa_K3187006</a>, <a href=" http://parts.igem.org/Part:BBa_K3187008 "
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                    target="_blank"> BBa_K3187008</a>
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</td>
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            </tr>
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            <tr>
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              <td>pSB1C3</td>
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              <td>Cmp</td>
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              <td>2070 bp</td>
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              <td>
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                CmR, lambda t0 terminator, high-copy-number
 +
                ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre-
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                and Suffix
 +
              </td>
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              <td><a href=" http://parts.igem.org/Part:BBa_K3187014 "
 +
                    target="_blank"> BBa_K3187014</a>, <a href=" http://parts.igem.org/Part:BBa_K3187015 "
 +
                    target="_blank"> BBa_K3187015</a>
 +
</td>
 +
            </tr>
 +
            <tr>
 +
              <td>pTeTW3con2</td>
 +
              <td>Cmp</td>
 +
              <td>4555 bp</td>
 +
              <td>
 +
                cat promoter, CmR, p15A ori, lacI repressor + lacI promoter,
 +
                tetR/tetA promoter, tetR repressor, tet operator, T7 promoter +
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                lac operator, T7 terminator
 +
              </td>
 +
              <td><a href=" http://parts.igem.org/Part:BBa_K3187011"
 +
                    target="_blank"> BBa_K3187011</a>, <a href=" http://parts.igem.org/Part:BBa_K3187012"
 +
                    target="_blank"> BBa_K3187012</a>, <a href=" http://parts.igem.org/Part:BBa_K3187013"
 +
                    target="_blank"> BBa_K3187013</a>
 +
</td>
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            </tr>
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            <tr>
 +
           
 +
            </tr>
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          </table>
 +
        </div>
 +
      </div>
 +
        </div>
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    </div>
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    </div>
  
  

Revision as of 14:27, 27 September 2020



University Experts Former iGEMers Others

Backbones

For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
Name Resistance Size Features Used for the following parts
pACYCT2 Cmp 3975 bp cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7 promoter, lac operator, T7 terminator BBa_K3187000, BBa_K3187001, BBa_K3187003, BBa_K3187004
pDESTara2 Amp 4556 bp AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator promoter, lac operator, T7 terminator BBa_K3187010
pET24 Kan 5236 bp KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator BBa_K3187000, BBa_K3187002, BBa_K3187005, BBa_K3187006, BBa_K3187008
pSB1C3 Cmp 2070 bp CmR, lambda t0 terminator, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre- and Suffix BBa_K3187014, BBa_K3187015
pTeTW3con2 Cmp 4555 bp cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, tetR/tetA promoter, tetR repressor, tet operator, T7 promoter + lac operator, T7 terminator BBa_K3187011, BBa_K3187012, BBa_K3187013