Difference between revisions of "Team:TU Darmstadt/Partnership/Summary And Methods"
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<tr> | <tr> | ||
<td scope="row">Used Enzymes</th> | <td scope="row">Used Enzymes</th> | ||
| − | <td> | + | <td>laccases: CotA of <i>Bacillus subtilis</i> and CueO of <i>Escherichia coli</i> |
| − | <td> | + | <br>esterase: EreB of <i>Escherichia coli</i></br></td> |
| − | <td> | + | <td>Laccase of <i>Botrytis aclada</i> and marine unknown</td> |
| + | <td>Laccases of <i>Trametes versicolor</i> and <i>S. cyaneus</i> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Model Organism</th> | <td scope="row">Model Organism</th> | ||
| − | <td> | + | <td><i>B. subtilis</i> and <i>E. coli</i></td> |
| − | <td> | + | <td>green algae <i>Chlamydomonas reinhardtii</i> and <i>Escherichia coli</i> as comparison</td> |
| − | <td> | + | <td><i>Pichia pastoris</i> and <i>E. coli </i></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Special Technique</th> | <td scope="row">Special Technique</th> | ||
| − | <td> | + | <td>immobilization of the enzymes in the matrix of the <i>Bacillus subtilis</i> biofilm</td> |
| − | <td> | + | <td>laccase secretion and filtration before access to wastewater</td> |
| − | <td> | + | <td>Immobilization of the enzymes in a silica foam </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Tested Substrates</th> | <td scope="row">Tested Substrates</th> | ||
| − | <td> | + | <td>Laccases: |
| − | <td> | + | ABTS, Diclofenac |
| − | <td> | + | |
| + | <br>EreB: | ||
| + | Erythromycin, Azithromycin</br> | ||
| + | </td> | ||
| + | <td>ABTS</td> | ||
| + | <td>ABTS, Diclofenac, Carbamazepine | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Genetic Engineering</th> | <td scope="row">Genetic Engineering</th> | ||
| − | <td> | + | <td>Construct Assembly: Gibson Assembly |
| − | <td> | + | <br>Genomic Integration: Homologous Recombination</br> |
| − | <td> | + | </td> |
| + | <td><i>Chlamydomonas reinhardtii</i>: MoClo | ||
| + | <br><i>Escherichia coli</i>: ordered/ synthesized plasmid</br></td> | ||
| + | <td>1) Gibson Assembly | ||
| + | <br>2) Restriction digestion</br> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td scope="row">Fun Facts</th> | <td scope="row">Fun Facts</th> | ||
| − | <td> | + | <td></td> |
| − | <td> | + | <td>only team of RLP</td> |
| − | <td> | + | <td></td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
| − | + | <div style="display: flex;justify-content: center;"> | |
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2020/d/d5/T--TU_Darmstadt--CD-ROM.jpg" alt="figure" width="1200" height="300"> | ||
| + | <figcaption id="Figure#">Figure 3: Workflow Stuttgart </figcaption> | ||
| + | </figure> | ||
| + | </div> | ||
Revision as of 11:09, 4 October 2020
General Workflow
iGEM TU Darmstadt
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iGEM TU Kaiserslautern
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iGEM Stuttgart
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| Issue | Darmstadt | Stuttgart | Kaiserslautern |
|---|---|---|---|
| Used Enzymes | laccases: CotA of Bacillus subtilis and CueO of Escherichia coli
esterase: EreB of Escherichia coli |
Laccase of Botrytis aclada and marine unknown | Laccases of Trametes versicolor and S. cyaneus |
| Model Organism | B. subtilis and E. coli | green algae Chlamydomonas reinhardtii and Escherichia coli as comparison | Pichia pastoris and E. coli |
| Special Technique | immobilization of the enzymes in the matrix of the Bacillus subtilis biofilm | laccase secretion and filtration before access to wastewater | Immobilization of the enzymes in a silica foam |
| Tested Substrates | Laccases:
ABTS, Diclofenac
EreB: Erythromycin, Azithromycin |
ABTS | ABTS, Diclofenac, Carbamazepine |
| Genetic Engineering | Construct Assembly: Gibson Assembly
Genomic Integration: Homologous Recombination |
Chlamydomonas reinhardtii: MoClo
Escherichia coli: ordered/ synthesized plasmid |
1) Gibson Assembly
2) Restriction digestion |
| Fun Facts | only team of RLP |