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− | < | + | <b>Why did we talk to him?</b> |
+ | <br> | ||
+ | PhD Yunrong Chai is an assistant professor at the College of Science at the Northeastern University in Boston and an expert for microbial genomics and biofilm formation. | ||
+ | We talked to him about our ideas to engineer and improve our biofilm. | ||
+ | <br> | ||
+ | |||
+ | <b>Which information did we gather?</b> | ||
+ | <li style="text-align:justify">Concerning our plan to knockout the <i>sinR</i> gene of our <i>B. subtilis</i> to simplify the induction of the biofilm formation, | ||
+ | PhD Chai explained that this knockout will make our biofilm more robust. | ||
+ | The effects on other properties of our <i>B. subtilis</i> and our biofilm should be minor. </li> | ||
+ | <li style="text-align:justify">Endospores are not able to produce our degradation enzymes, which is why preventing sporulation of our <i>B. subtilis</i> would be useful. | ||
+ | As the sigma factor F (<i>σ<sup>F</sup></i>) regulates genes associated with sporulation, he suggested a <i>σ<sup>F</sup></i> knockout to avoid sporulation. | ||
+ | PhD Chai predicted no impact on gene expression outside of sporulation. </li> | ||
+ | <li style="text-align:justify">The combination of a <i>σ<sup>F</sup></i> and <i>sinR</i> knockout would lead to a <i>B. subtilis</i> that can not sporulate and forms a more robust biofilm. </li> | ||
+ | <li style="text-align:justify">A fusion protein of the matrix protein TasA with our enzymes would be a perfect way to display the degradation enzymes in the biofilm matrix. | ||
+ | Because TasA is very abundant, many enzymes would be located in the matrix. | ||
+ | Furthermore, he successfully generated fusion proteins of TasA with fluorescence proteins in his lab.</li> | ||
+ | <li style="text-align:justify">A mixed biofilm of different microorganisms could increase the robustness of our biofilm but would also complicate our assays and <b>would be an advanced step in your project.<b> </li> | ||
+ | |||
+ | <br> | ||
+ | |||
+ | <b>How did we adjust our project?</b> | ||
+ | We continued planning our fusion proteins with TasA and the knockout of <i>sinR</i>. Additionally, we integrated the knockout of <i>σ<sup>F</sup></i> into our project to avoid sporulation of our <i>B. subtilis</i>. | ||
+ | <br><br> | ||
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Revision as of 13:48, 10 October 2020
Which information did we gather?
How did we adjust our project?
text über Prof. Dr. Jörg Oehlmann
Synthetic Biology
What information did we gather?
How did we adjust our project?
bla bla
PhD Yunrong Chai is an assistant professor at the College of Science at the Northeastern University in Boston and an expert for microbial genomics and biofilm formation. We talked to him about our ideas to engineer and improve our biofilm.
Which information did we gather?
How did we adjust our project? We continued planning our fusion proteins with TasA and the knockout of sinR. Additionally, we integrated the knockout of σF into our project to avoid sporulation of our B. subtilis.
Implementation
What information did we gather?
How did we adjust our project?
For a more detailed review on her input, please CLICK HERE.
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Since our project is to be integrated into wastewater treatment plants in the future, we wanted to have a first-hand look at such a plant. For that, we visited the wastewater treatment plant from ENTEGA in Darmstadt. This visit gave us the opportunity to see by ourselves how wastewater treatment can work and which steps are necessary to achieve a clean outcome. We learned how different waste water treatment plants can be and how differently they have to deal with new guidelines concerning the quality of the treated wastewater. The wastewater treatment plant in Darmstadt is built of a primary treatment clarifier, followed by a biological wastewater treatment and a final clarifier.
For the implementation of our biofilm, a new clarifier for an additional purification stage would be necessary in the mentioned wastewater treatment plant. Space for another tank in this plant is available but we have to consider that especially wastewater treatment plants in bigger cities wouldn’t have enough space for this. Considering this issue, we thought about different options to implement our project (link Biofilm in chemischer Stufe, Filmis).
Ethics
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