Team:TU Darmstadt/Parts

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Basic Parts Composite Parts in vitro characterization immobilization on our biofilm our kill switch

Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we refrained from uploading them to the registry as there exists no primary literature on them, but we still provide schematic images on them.

Basic Parts

Due to Covid-19 we were not able to test any of our designed parts in the lab. Nevertheless, we registered our basic parts, so future teams are easily able to characterize them. For our Composite Parts we refrained from uploading them to the registry as there exists no primary literature on them, but we still provide schematic images on them. All our basic parts are depicted in the table below. Because we were not able to conduct any lab work , we did not submit any parts for the special prizes Best Basic Part.
Part(BBa_) Type Description Length in bp
BBa_K3429000 Promotor pgrac promotor 170
BBa_K3429001 Coding Sequence TasA matrix protein 783
BBa_K3429002 Coding Sequence Otto @mdo
BBa_K3429003 Coding Sequence Otto @mdo
BBa_K3429004 Coding Sequence Otto @mdo
BBa_K3429005 Coding Sequence Otto @mdo
BBa_K3429006 Coding Sequence Otto @mdo
BBa_K3429007 Coding Sequence Otto @mdo
BBa_K3429008 Coding Sequence Otto @mdo
BBa_K3429009 Promotor Otto @mdo
BBa_K3429011 Coding Sequence Otto @mdo
BBa_K3429012 Coding Sequence Otto @mdo
This could be your part number Coding Sequence Otto @mdo

Composite Parts

For in vitro characterization

For purification and in vitro characterization of our enzymes we designed the following Composite Parts : cotA, cueO, and ereB. All of these Composite Parts contain a strep-tag for purification and are optimized for E. coli.
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Figure 1: Composite parts for in vitro characterization of our enzymes

For Immobilization on our biofilm

The following parts were designed for immobilization of our enzymes on the TasA matrix protein of our B. subtilis biofilm. In these composite parts the enzymes are genetically fused to TasA .
Figure 2: Composite parts for immobilization on biofilm matrix

For our kill switch

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Registry Parts


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