Team:XMU-China/Parts

This year, among the 41 parts designed, ten are basic parts and the other are composite ones. All of the parts are designed by ourselves, and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).

Among the ten basic parts designed this year by ourselves, BBa_K2922040 is our favorite one, which comes from human genomes. The gene enables engineering bacteria to form a vesicle which will have great applications in the enrichment of substrate (such as metal ions) through extracellular membrane and the enhancement of cell stress resistance. Besides, many other basic parts have also been designed to construct our project.

Favorite	Name	Type	Description	Designer	Length
R	BBa_K3332011	Coding	GRHPR-his-tag	Xiaoyu Wang	1005
	BBa_K3332000	Coding	iNAP-his-tag	Shuling Xiao	1167
	BBa_K3332001	Coding	His-tag-iNAP	Shuling Xiao	1167
	BBa_K3332002	Coding	INPNC-iNAP	Shuling Xiao	2128
	BBa_K3332003	Coding	Lpp-OmpA-iNAP	Shuling Xiao	1614
	BBa_K3332004	Coding	iNAP-AIDA	Shuling Xiao	2631
	BBa_K3332005	Coding	iNAP-BrkA	Shuling Xiao	2547
	BBa_K3332006	Coding	GOX-his-tag	Xiaoyu Wang	951
	BBa_K3332007	Coding	INPNC-GOX	Xiaoyu Wang	1908
	BBa_K3332008	Coding	Lpp-OmpA-GOX	Xiaoyu Wang	1398
	BBa_K3332009	Coding	GOX-AIDA	Xiaoyu Wang	2415
	BBa_K3332010	Coding	GOX-BrkA	Xiaoyu Wang	2331
	BBa_K3332012	Coding	INPNC-GRHPR	Shi Zhang	1962
	BBa_K3332013	Coding	Lpp-OmpA-GRHPR	Shi Zhang	1452
	BBa_K3332014	Coding	GRHPR-AIDA	Shi Zhang	2469
	BBa_K3332015	Coding	GRHPR-BrkA	Shi Zhang	2385
	BBa_K3332016	Coding	INPNC-GFP	Shi Zhang	1695
	BBa_K3332017	Coding	Lpp-OmpA-GFP	Shi Zhang	1185
	BBa_K3332018	Coding	GFP-AIDA	Yixian Zheng	2199
	BBa_K3332019	Coding	GFP-BrkA	Yixian Zheng	2252
	BBa_K3332020	Coding	phnE₁	Yixian Zheng	963
	BBa_K3332021	Coding	phnE₂	Yixian Zheng	1518
	BBa_K3332022	Coding	phnC	Yixian Zheng	840
	BBa_K3332023	Coding	phnD	Yixian Zheng	906
	BBa_K3332024	Coding	phnJ	Zhichun Yang	846
	BBa_K3332025	Coding	phnJ_mut21	Zhichun Yang	846
	BBa_K3332026	Coding	phnJ_mut40&16	Zhichun Yang	846
	BBa_K3332027	Coding	phnJ_mut45	Zhichun Yang	846
	BBa_K3332028	Coding	phnO-his-tag	Jisheng Xie	483
	BBa_K3332029	DNA	OmpA 5'UTR-phnF 0.97-Hfq binding sequence	Zhichun Yang	223
	BBa_K3332030	DNA	OmpA 5'UTR-phnF 0.85-Hfq binding sequence	Ruomeng Bi	223
	BBa_K3332031	DNA	OmpA 5'UTR-phnF 0.75-Hfq binding sequence	Ruomeng Bi	223
	BBa_K3332032	DNA	OmpA 5'UTR-phnJ 0.94-Hfq binding sequence	Ruomeng Bi	223
	BBa_K3332033	DNA	OmpA 5'UTR-phnJ 0.69-Hfq binding sequence	Ruomeng Bi	223
	BBa_K3332034	DNA	PLtetO promoter	Ruomeng Bi	142
	BBa_K3332035	Coding	E0020-ssrAtag（mf-lon）	Ruomeng Bi	819
	BBa_K3332036	RBS	RBS1	Jinzhu Mao	9
	BBa_K3332037	Coding	LacI-ssrAtag(mf-lon)	Jinzhu Mao	1185
	BBa_K3332038	DNA	Ptrc-2 promoter	Jinzhu Mao	91
	BBa_K3332039	Coding	tetR	Jinzhu Mao	624
	BBa_K3332040	DNA	Ptrc-2 derivative promoter	Ruomeng Bi	130
	BBa_K3332042	DNA	Formaldehyde_derivative-1 promoter	Jisheng Xie	581
	BBa_K3332043	DNA	Formaldehyde_derivative-2 promoter	Zinuo Huang	645
	BBa_K3332044	DNA	Formaldehyde_derivative-3 promoter	Xingyu Chen	631

This year, we have formulated a Part Collection related to secretion of intracellular proteins, which includes two major parts: one is secretion achieved with the help of YebF in the form of fusion proteins, the other is the expression of Kil protein by promoters of different intensities, thus changing cell permeability to different degrees and finally realizing secretion. For our Part Collection, the efficiency of three different intracellular proteins we utilized were well tested, recorded in detail and completely uploaded to the registry, which would be instructive in the field of design and characterization for many teams.

Register Name	Code used in Lab	Description	Types	Lenth
BBa_K2922000	y-bgl1A	The protein beta-glucosidase with protein YebF fused to its N-terminus by GS Linker.	Coding	1764
BBa_K2922001	y-cenA	The protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker.	Coding	1731
BBa_K2922002	y-cex	The protein exoglucanase with protein YebF fused to its N-terminus by GS Linker.	Coding	1839
BBa_K2922009	109-kil	The expression of Kil protein by J23109 promoter	Composite	248
BBa_K2922010	112-kil	The expression of Kil protein by J23112 promoter	Composite	248
BBa_K2922011	114-kil	The expression of Kil protein by J23114 promoter	Composite	248
BBa_K2922012	109-kil-T7-bgl1A	The secretion of beta-glucosidase by BBa_K2922009.	Composite	1760
BBa_K2922013	112-kil-T7-bgl1A	The secretion of beta-glucosidase by BBa_K2922010.	Composite	1760
BBa_K2922014	114-kil-T7-bgl1A	The secretion of beta-glucosidase by BBa_K2922011.	Composite	1760
BBa_K2922015	109-kil-T7-cenA	The secretion of endoglucanase by BBa_K2922009.	Composite	1727
BBa_K2922016	112-kil-T7-cenA	The secretion of endoglucanase by BBa_K2922010.	Composite	1727
BBa_K2922017	114-kil-T7-cenA	The secretion of endoglucanase by BBa_K2922011.	Composite	1727
BBa_K2922018	109-kil-T7-cex	The secretion of exoglucanase by BBa_K2922009.	Composite	1835
BBa_K2922019	112-kil-T7-cex	The secretion of exoglucanase by BBa_K2922010.	Composite	1835
BBa_K2922020	114-kil-T7-cex	The secretion of exoglucanase by BBa_K2922011.	Composite	1835