Team:TU Darmstadt/Project/Backbones
For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
| Name | Size | Resistance | Features | Organism |
|---|---|---|---|---|
| pET24(+) | 5236 bp | Kanamycin | KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator | E. coli |
| pSEVA3b67Rb | 4617 bp | Chloramphenicol | high-copy-number pUC ori, pTHT15 ori, repB, mRFP1, rrnB T1 terminator, oriT, lambda t0 terminator | E. coli, B. subtilis |
| pJET1.2 | 2974 bp | Ampicillin | AmpR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori | E. coli |