Team:TU Darmstadt/Project/Backbones
Backbones
For the in silico design of our experiments for the wetlab we used several different plasmid backbones. Due to Covid-19 we were not able to test any of our constructs in the lab. Therefore, we only planned the shown backbones theoretically. The plasmids would be used for cloning, expression, and genomic integration of our constructs. You can find a list of all plasmids we used for our design and the corresponding parts below.
| Name | Resistance | Size | Features | Used for the following parts |
|---|---|---|---|---|
| pACYCT2 | Cmp | 3975 bp | cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7 promoter, lac operator, T7 terminator | BBa_K3187000, BBa_K3187001, BBa_K3187003, BBa_K3187004 |
| pDESTara2 | Amp | 4556 bp | AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator promoter, lac operator, T7 terminator | BBa_K3187010 |
| pET24 | Kan | 5236 bp | KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator | BBa_K3187000, BBa_K3187002, BBa_K3187005, BBa_K3187006, BBa_K3187008 |
| pSB1C3 | Cmp | 2070 bp | CmR, lambda t0 terminator, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre- and Suffix | BBa_K3187014, BBa_K3187015 |
| pTeTW3con2 | Cmp | 4555 bp | cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, tetR/tetA promoter, tetR repressor, tet operator, T7 promoter + lac operator, T7 terminator | BBa_K3187011, BBa_K3187012, BBa_K3187013 |