Which information did we gather?
How did we adjust our project?
text über Prof. Dr. Jörg Oehlmann
Synthetic Biology
What information did we gather?
How did we adjust our project?
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PhD Yunrong Chai is an assistant professor at the College of Science at the Northeastern University in Boston and an expert for microbial genomics and biofilm formation. We talked to him about our ideas to engineer and improve our biofilm.
Which information did we gather?
How did we adjust our project? We continued planning our fusion proteins with TasA and the knockout of sinR. Additionally, we integrated the knockout of σF into our project to avoid sporulation of our B. subtilis.
Implementation
What information did we gather?
How did we adjust our project?
For a more detailed review on her input, please CLICK HERE.
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In order to implement our biofilm into wastewater treatment plants (WWTP) we have to deal with legal requirements in Germany. Therefore, the team from Kaiserslautern had the idea to contact Dr. Ulrich Ehlers from the Federal Office for Consumer Protection and Food Safety (BVL) and since we are working close together, we were able to ask him a few questions. He has been the head of the "release and placing on the market" section in the BVL’s "Genetic Engineering" department.
Which information did we gather?
How did we adjust our project?
We know whom we have to contact, if we plan to implement our system into the first WWTP. We have also put our focus more on designing a kill switch, which would help us working in content use and getting a WWTP classified as genetic engineering plant
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Since our project is to be integrated into wastewater treatment plants in the future, we wanted to have a first-hand look at such a plant. For that, we visited the wastewater treatment plant from ENTEGA in Darmstadt. This visit gave us the opportunity to see by ourselves how wastewater treatment can work and which steps are necessary to achieve a clean outcome. We learned how different waste water treatment plants can be and how differently they have to deal with new guidelines concerning the quality of the treated wastewater. The wastewater treatment plant in Darmstadt is built of a primary treatment clarifier, followed by a biological wastewater treatment and a final clarifier.
For the implementation of our biofilm, a new clarifier for an additional purification stage would be necessary in the mentioned wastewater treatment plant. Space for another tank in this plant is available but we have to consider that especially wastewater treatment plants in bigger cities wouldn’t have enough space for this. Considering this issue, we thought about different options to implement our project (link Biofilm in chemischer Stufe, Filmis).
Ethics
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