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Background

With the development of technology these days, the production of grains is increasing annually. As a result, increasing amount of substandard grains is causing a big deal as large amount of resources is wasted every year.

Goal

Turning Waste into Treasure: Reuse of substandard grains as fermentation substrate to produce biodiesel.

Abstract

When we are here & Lofty Goal

Substandard grains, such as long-term stored grains, cannot be eaten due to the existed toxin and contaminants. Therefore, these grains attracted much attention as the fermentation substrate. Meanwhile, the biodiesel attracted increasing attention as a promising biofuel to replace fossil diesel for the last two decades. In this work, Yarrowia lipolytica has been selected as the model host for biodiesel production.

Taking into account the disability to degrade the raw starch of Y. lipolytica, we separately express heterologous alpha‑amylase and glucoamylase enzymes in it. The results showed that the strains expressing glucoamylase were able to grow on starch as sole carbon source. By optimizing the C/N=100, the total lipid content increases up to 21 % of DCW, which sets up the basis for further studies.

In the future, we aim to provide a practical, economical, and environment-friendly method to treat substandard grains and produce biodiesel.

Expert Team

Our Team

Far far away, behind the word mountains, far from the countries Vokalia and Consonantia, there live the blind texts. Separated they live in

Zihan. Tian

Team Leader

I am an ambitious workaholic, but apart from that, having an artist's soul.

Xinhe.Shen

Team Leader

As a student, I consider reading an important quality, for it brings me knowledge.

Jianpu.Gao

Laboratorian

Exercise is important for physical health, and it increases my efficience in the lab.

Yiran.Xu

Media people

Passing knowledge to other is the best art in this world, and it is essential for the civilization to development.

Team:Worldshaper-Nanjing