Team:AHUT-ZJU-China/Characterization
AHUT-ZJU-China
Characterization
READING TIME: 63mins
In 2020, AHUT-China iGEM team has characterized the output of this part in another chassis E. coli TB1. The result was documented in the experience page and the main page of BBa_K2547000.
The sequence of BBa_K2547000 was synthesized and cloned it into the pET-30a(+) expression plasmid to obtain the recombinant expression vector, and then we characterized that this part could be expressed in another strain TB1, as shown in Figure 1.
Fig. 1 SDS-PAGE analysis for CA2 cloned in pET-30a(+) and expressed in TB1 strain