Team:BGU-Israel/protocol

General

General protocols used on daily basis:

  • Antibiotic stock preparation
  • LB agar plates preparation
  • DH5a competent cell transformation

Plasmids

Protocols used for plasmids' construction. All experiments were carried out with Escherichia coli DH5-alpha strains:

  • Mini-prep kit – plasmid extraction from bacteria cells
  • KAPA HiFi HotStart ReadyMix PCR kit
  • DNA electrophoresis
  • Gel extraction
  • Gibson assembly

Activity assays

Protocols used for cellulase enzyme activity assays:

  • Cellulase E1 characterization
  • Cellulolytic complex characterization
  • Growing bacteria with CMC as carbon source

Address

Ben-Gurion Univesity of the Negev
Ben Gurion 1, Beer Sheva 8410501, Israel

Ben Gurion University

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