Team:BNU-China/Attributions
Introduction
This year, our team has 31 members, which are divided into 4 groups: wet lab group, modeling group,
wiki group and art design group to work effectively. Our idea was raised up through brainstorms. During our experiments,
we created several parts to prove our concept and the idea was improved by Human Practice.
Team Attributions
Wet Lab
Our wet lab experiment started on August 21st 2020. We divided 20 wet lab members into 3 groups to achieve different parts of our design.
Ran Yan, Yiying Yu, Xinrui Li, Aoyu Ma, Xinwen Dong, Yuxin Wang,Yumo Li, students of BNU, constructed recombinant plasmids, electroporated S.cerevisiae, and synchronized it. Then, they used western blot to get the Cas9 protein levels over time, which proved the possibility of expressing Cas9 periodically.
Minglei Zhang, Jiangyue Zhang, Yunhan Jiao, Xinmiao Li, Mingyue Hu, Xinyue Shan, Yuzhen Chen, students of BNU, used GAL1 promoter to express the first 124 amino acids of Clb2 and GFP. Combining with modeling, they characterized the degradation effect of the first 124 amino acids of Clb2. It is shown that Cas9 can be degraded before early G1 phase, ensuring that parents and offspring have different barcodes.
Yiling Chen, Chang Gao, Xinyao Pan, Xiaoxue Zhao, Fanmeng Zhang, Jingzi Wang, Students of BNU, designed and characterized the double promoter system, successfully proved that our project can obtain the lineage information out of transcriptomic information.
Model
Xiaoyu Ye, Liang Li, Lin Xu, Nianci Jiang, students of BNU, constructed Cas9 folding rate model, which demonstrated the feasibility of our project preliminary.
Xiaoyu Ye, Liang Li, Lin Xu, constructed the degradation rate model, proving that it can meet our requirements.
Xiaoyu Ye, Liang Li, Lin Xu, Nianci Jiang, constructed a model to predict the total number of barcode produced by hgRNA. It is demonstrated that by using hgRNA, the diversity is improved greatly.
Nianci Jiang, Xiaoyu Ye, constructed a model to guide potential users on how many hgRNA should be used in different conditions.
Xiaoyu Ye, Liang Li, Lin Xu, constructed the degradation rate model, proving that it can meet our requirements.
Xiaoyu Ye, Liang Li, Lin Xu, Nianci Jiang, constructed a model to predict the total number of barcode produced by hgRNA. It is demonstrated that by using hgRNA, the diversity is improved greatly.
Nianci Jiang, Xiaoyu Ye, constructed a model to guide potential users on how many hgRNA should be used in different conditions.
Art
Qing Hu, Huilin Chen, Yuxin Wang, students of BNU, worked on the art of the project, including logos, posters, team uniform design, and team-related products. What's more, they dedicated to artworks in videos, human practices and wiki.
Pengbo Liu, students of BNU, was in charge of planning, shooting, editing and post-production in promotion video and presentation video.
Pengbo Liu, students of BNU, was in charge of planning, shooting, editing and post-production in promotion video and presentation video.
Wiki
Yuchen Feng, Dake Gao, Shibei Meng, Jingyuan Wang, students of BNU, designed and completed the wiki structure, connected with other groups and collected wiki information, inserted wiki dynamic effects, adjusted the static art design of wiki, uploaded contents to wiki.
Human Practice
Minglei Zhang, Yiying Yu, Jiangyue Zhang, interviewed Zhen Xie on barcode diversity.
Yiling Chen, Xinyao Pan, Yuzhen Chen, interviewed Guangdun Peng about the necessity to read lineage information at RNA level.
Jiangyue Zhang, Yiling Chen, Minglei Zhang, communicated with technical personnel of Novogene to know more about 10X Genomics platforms.
Ran Yan, Yiling Chen, Yumo Li, Jingzi Wang, conducted online courses.
Jingzi Wang, Xinyao Pan, Yuzhen Chen, Ran Yan, made popular science manual and sent them to the public.
Xinwen Dong, managed WeChat iGEM official account of Beijing Normal University.
Yiling Chen, Xinyao Pan, Yuzhen Chen, interviewed Guangdun Peng about the necessity to read lineage information at RNA level.
Jiangyue Zhang, Yiling Chen, Minglei Zhang, communicated with technical personnel of Novogene to know more about 10X Genomics platforms.
Ran Yan, Yiling Chen, Yumo Li, Jingzi Wang, conducted online courses.
Jingzi Wang, Xinyao Pan, Yuzhen Chen, Ran Yan, made popular science manual and sent them to the public.
Xinwen Dong, managed WeChat iGEM official account of Beijing Normal University.
Project Support and Advice
Jinbo Chen, primary PI, offered us advice and financial support, organized BNU-China.
Xiaoran Hao, secondary PI, conducted laboratory safety training, provided the team with experiment and equipment support, managed our laboratory space, and taught us the techniques used in the development of the biological part.
Prof. Xudong Zhu, Prof. Dong Yang, instructor from Beijing Normal University, provided inspiration and guidance with general suggestions on all aspects, and helped optimize experiments and teaching materials.
Xiaoran Hao, secondary PI, conducted laboratory safety training, provided the team with experiment and equipment support, managed our laboratory space, and taught us the techniques used in the development of the biological part.
Prof. Xudong Zhu, Prof. Dong Yang, instructor from Beijing Normal University, provided inspiration and guidance with general suggestions on all aspects, and helped optimize experiments and teaching materials.
Advisor
Yiyun Zhang, Zhe Feng, advisors of BNU-China, gave us experimental training, made suggestions of the whole project.
External Support
Human practice support / Project advice
Jiekai Chen, principle investigator of Guangzhou Institutes of Biomedicine and Health, pointed out the advantages and disadvantages about lineage tracing technology based on CRISPR/Cas9.
Junjiu Huang, professor of Sun Yat-sen University, told us the biggest problem of this technology is the limited barcodes.
Jie Na，associate professor of Center for Stem Cell Biology, reminded us of the safety and ethical issues that may be involved in the technology.
Zhen Xie, professor of Department of Automation, and chunlai Chen, professor of Tsinghua University, gave us some suggestions on experiments.
Guangdun Peng, Professor of Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, told us that it is necessary to read the lineage tracing information at the RNA level.
Technical personnel of Novogene, introduced 10X Genomics platforms to us in detail.
Junjiu Huang, professor of Sun Yat-sen University, told us the biggest problem of this technology is the limited barcodes.
Jie Na，associate professor of Center for Stem Cell Biology, reminded us of the safety and ethical issues that may be involved in the technology.
Zhen Xie, professor of Department of Automation, and chunlai Chen, professor of Tsinghua University, gave us some suggestions on experiments.
Guangdun Peng, Professor of Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, told us that it is necessary to read the lineage tracing information at the RNA level.
Technical personnel of Novogene, introduced 10X Genomics platforms to us in detail.
General Support
Thanks for the support from the College of Life Science, Beijing Normal University.
Chenxi Li, student of BNU, offered us great support throughout the whole project on giving guidance and advice on doing experiments.
Chenxi Li, student of BNU, offered us great support throughout the whole project on giving guidance and advice on doing experiments.
Lab Support
The College of Life Science, Beijing Normal University, provided us the equipment, reagents and expenses.
Fengyan Bai, professor of Institute of Microbiology, Chinese Academy of Sciences;
Xia Ke，associated professor of college of Biotechnology and Bioengineering, Zhejiang University of Technology;
Qinhong Wang, Professor, Associate director, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences;
Yuping Li, Associate professor, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xinxiao Sun, Associate Professor of Beijing University of Chemical Technology provided us strain BY4741.
Weifeng Liu, Professor of Molecular Microbiology, State Key Laboratory of Microbial Technology Institute of Microbial Biotechnology, Shandong University and Hui Liu from Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences provided us pYES2 plasmid.
Fengyan Bai, professor of Institute of Microbiology, Chinese Academy of Sciences;
Xia Ke，associated professor of college of Biotechnology and Bioengineering, Zhejiang University of Technology;
Qinhong Wang, Professor, Associate director, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences;
Yuping Li, Associate professor, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Xinxiao Sun, Associate Professor of Beijing University of Chemical Technology provided us strain BY4741.
Weifeng Liu, Professor of Molecular Microbiology, State Key Laboratory of Microbial Technology Institute of Microbial Biotechnology, Shandong University and Hui Liu from Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences provided us pYES2 plasmid.
Acknowledgement
Thanks for all the people who provided help during our project.
Thanks for The College of Life Science, Beijing Normal University and teachers in our college to give us all round help and careful guidance.
Thanks for all the people who gave advice to refine the project and took part in our Human Practice activities.
Thanks for iGEM foundation, Conference of China iGEMer Community for the platform for communication.
Thanks for The College of Life Science, Beijing Normal University and teachers in our college to give us all round help and careful guidance.
Thanks for all the people who gave advice to refine the project and took part in our Human Practice activities.
Thanks for iGEM foundation, Conference of China iGEMer Community for the platform for communication.
Contact
Address:19th,Xinjiekouwai St, Haidian District, Beijing, P.R.China
Postcode:100875
Email：igem2020bnu_china@163.com
Postcode:100875
Email：igem2020bnu_china@163.com
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