Team:DTU-Denmark/Experiments

Experiments

The different steps of genetic modification of A. niger are given in the flowsheet below. The protocol for each step a can be downloaded below.



Click on the protocols below to download them.


PCR amplification of Biobricks

Gel electrophoresis and Gel purification

PacI & Nt. BbvCI digestion of backbone

USER cloning of Biobricks and plasmid

Transform into E. coli

Make E. coli competent cells

Screen transformants through Colony PCR




Purification of plasmids

Grow A. niger on plates

Make protoplasts

Transform plasmid into A. niger

Screen transformants through Tissue PCR

Monosporic purification

Make a stock

Protocols specific for our project

Sample preparation for microscope slides

Glucoamylase Assay

Bioreactor
BCA Assay

BioLector

DNA extraction


Some of these protocols have been adapted from our lab at DTU bioengineering, and others we have developed ourselves through the course of this project.