Team:DTU-Denmark/Part Collection



Our morphology toolbox for Aspergillus niger

We have made an extensive toolbox for making morphological changes in our model organism. Here is a collection of CRISPR-Cas9 parts that can be used to engineer morphology. The present collection comprises 40 parts for knocking out 7 genes related to morphology, which can be combined in endless ways to help engineer morphology in the future. The RESHAPE team present the Morphology Part Collection.



Part name Part number Type
pFC330 BBa_K3385000 Plasmid (15724 bp)
pFC902 BBa_K3385001 Plasmid (16614 bp)
Aspergillus nidulans glycin tRNA BBa_K3385002 RNA (71 bp)
gRNA backbone BBa_K3385003 RNA (80 bp)
Aspergillus fumigatus U3 promoter BBa_K3385004 Regulatory (468 bp)
Aspergillus fumigatus U3 terminator BBa_K3385005 Terminator (226 bp)
Ptef BBa_K3385006 Regulatory (886 bp)
Aspergillus niger Cas9 BBa_K3385007 Coding (4131 bp)
crRNA_chsC_KO_up BBa_K3385012 RNA (20 bp)
crRNA_chsC_KO_down BBa_K3385013 RNA (20 bp)
CRISPR_chsC_KO BBa_K3385014 Composite (1107 bp)
chsC_repair_oligo BBa_K3385015 Primer (90 bp)
crRNA_spaA_KO_up BBa_K3385016 RNA (20 bp)
crRNA_spaA_KO_down BBa_K3385017 RNA (20 bp)
CRISPR_spaA_KO BBa_K3385018 Composite (1107 bp)
spaA_repair_oligo BBa_K3385019 Primer (90 bp)
crRNA_gul-1_KO_up BBa_K3385020 RNA (20 bp)
crRNA_gul-1_KO_down BBa_K3385021 RNA (20 bp)
CRISPR_gul-1_KO BBa_K3385022 Composite(1107 bp)
gul-1_repair_oligo BBa_K3385023 Primer (90 bp)
crRNA_arfA_KO_up BBa_K3385024 RNA (20 bp)
crRNA_arfA_KO_down BBa_K3385025 RNA (20 bp)
CRISPR_arfA_KO BBa_K3385026 Composite (1107 bp)
arfA_repair_oligo BBa_K3385027 Primer (90 bp)
crRNA_racA_KO_up BBa_K3385028 RNA (20 bp)
crRNA_racA_KO_down BBa_K3385029 RNA (20 bp)
CRISPR_racA_KO BBa_K3385030 Composite (1107 bp)
racA_repair_oligo BBa_K3385031 Primer (90 bp)
crRNA_aplD_KO_up BBa_K3385032 RNA (20 bp)
crRNA_aplD_KO_down BBa_K3385033 RNA (20 bp)
CRISPR_aplD_KO BBa_K3385034 Composite (1107 bp)
aplD_repair_oligo BBa_K3385035 Primer (90 bp)
crRNA_pkaR_KO_up BBa_K3385036 RNA (20 bp)
crRNA_pkaR_KO_down BBa_K3385037 RNA (20 bp)
CRISPR_pkaR_KO BBa_K3385038 Composite (1107 bp)
pkaR_repair_oligo BBa_K3385039 Primer (90 bp)
pyrG BBa_K3385072 Coding (1543 bp)
E_coli_backbone BBa_K3385073 Plasmid_Backbone (2908 bp)
NLS BBa_K3385074 Tag (24 bp)
PacI/Nt.BbvCI cassette BBa_K3385075 Other (26 bp)