Team:Exeter/Notebook/August6
Thursday, 6 August
Lab Work
Due to lack of lab access, those of us sharing accommodation (who did not need to socially distance due to living together) set up a makeshift lab in our kitchen, consisting of a shaking incubator and spectrophotometer as well as pipettes/lab coats/goggles/gloves etc. We conducted an experiment to test the efficiency of two new Bacillus subtilis promoters, hoping that we would be able to characterise a new part for the iGEM registry.
We made up a solution of:
- 937.5 ul B. subtilis CFE
- 937.5 ul amino acid mix (AA)
- 262.5 ul energy mix
- 262.5 ul S30A buffer
- 225 ul PEG8000
One B. sub culture had a plasmid with the gene for blue chromoprotein amilCP and the other had a plasmid encoding red fluorescent protein meffCP.
We plated the solution as follows:
We placed the well plate in the shaking incubator and measured it in the spectrophotometer at 2 hour intervals for about 8 hours. We checked it once again at 24 hours after first incubation.
Unfortunately our experiment was unsuccessful (the well plates failed to show any colour and the spectrophotometer didn't pick up any significant changes) so we repeated it again on the 7th.
Science Communication and Outreach
We posted an infographic about the applications of CaCO3 precipitated by bacteria.