Team:Exeter/Notebook/Sept10
Thursday, 10 September
Anna's housemate Ed Hollis who studies Engineering designed us a preliminary 3D printer nozzle with two inputs (one for the gel and one for the bacteria). The inputs intercept at different planes which creates mixing within the nozzle itself and avoids the need for a mixing chamber that would require regular cleaning. The nozzle is detachable and reusable:
Lab Work
We attempted the B. sub transformations a third time. This time while incubating the B. sub and measuring it at 20 minute intervals, the spectrometer did not show any growth at all. We decided to abort the experiment.
Our supervisor Mark had advised us previously that if B. sub could not be successfully transformed, the most time-efficient thing to do would be to express our proteins in E. coli and then conduct experiments on the proteins after extracting them with an assay.
Adam and Matthew went into the lab to clone the 12 plasmids we needed for E. coli transformations, however it turned out we didn't have any plasmids left. Instead, we decided to miniprep the px1834 plasmids on Friday by extracting them from a stock of non-transformed E. coli.
Team Collaborations
We had a meeting with UCL (with supervisors from both teams present) to discuss the wet lab work we were going to do for them as part of our collaboration.
UNSW emailed us promotional material for the Coral Symposium so that we could share it on social media.