Team:HKUST/Composite Parts

• Project
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  • Design
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• Dry Lab
  • Protein Structure Pretiction
  • Molecular Dynamics Simulation
  • Color Mixing
• Wet Lab
  • Description
  • Cloning
  • Expression & Purification
  • Thread Formation
  • Color Mixing
  • Draw-Processing
• Human Practice
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  • Composite Parts
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Composite Parts

• Composite Parts

Composite Parts

1. BBa_K3581006: This composite part includes composite part BBa_K880005, the alpha subunit BBa_K3581000, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and terminator BBa_B0015. The aim of this part is to maximize protein expression of the alpha subunit of hagfish intermediate filaments through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

2. BBa_K3581007: This composite part includes composite part BBa_K880005, the gamma subunit BBa_K3581001, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and terminator BBa_B0015. The aim of this part is to maximize protein expression of the gamma subunit of hagfish intermediate filaments through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

3. BBa_K3581008: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, the alpha subunit BBa_K3581000, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting alpha subunit of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

4. BBa_K3581009: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, the gamma subunit BBa_K3581001, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting gamma subunit of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

5. BBa_K3581010: This composite part includes composite part BBa_K880005, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and terminator BBa_B0015. The aim of this part is to maximize protein expression through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

6. BBa_K3581011: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, His tag BBa_K3581002, HlyA signal peptide BBa_K3581003 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting subunits of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

7. BBa_K3581015: This composite part includes composite part BBa_K880005, the alpha subunit BBa_K3581000, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and terminator BBa_B0015. The aim of this part is to maximize protein expression of the alpha subunit of hagfish intermediate filaments through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

8. BBa_K3581016: This composite part includes composite part BBa_K880005, the gamma subunit BBa_K3581001, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and terminator BBa_B0015. The aim of this part is to maximize protein expression of the gamma subunit of hagfish intermediate filaments through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

9. BBa_K3581017: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, the alpha subunit BBa_K3581000, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting alpha subunit of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

10. BBa_K3581018: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, the gamma subunit BBa_K3581001, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting gamma subunit of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

11. BBa_K3581019: This composite part includes composite part BBa_K880005, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and terminator BBa_B0015. The aim of this part is to maximize protein expression through the use of strong promoters and RBS optimal for protein secretion. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a double terminator which is commonly used for termination.

12. BBa_K3581020: This composite part includes T7 promoter BBa_I712074, RBS BBa_B0030, His tag BBa_K3581002, TorA signal peptide BBa_K3581014 and T7 terminator BBa_B0016. The aim of this part is to replicate results of a previous experimental data which also used T7 parts by secreting subunits of hagfish intermediate filaments. The His tag will be used for purifying the protein and TEV protease cut site can cleave out the tag once the protein is purified. The signal peptide is flanked by HindIII cut sites which will allow us to secrete the protein extracellularly, when we can also cut out the signal peptide using HindIII to secrete them as inclusion bodies to determine the best method with highest yield. The terminator is a T7 terminator which is commonly paired with T7 promoters.

References

1. http://parts.igem.org/Part:BBa_K1518001 (retrieved on 10/10/2020)
2. http://parts.igem.org/Part:BBa_K1518000 (retrieved on 10/10/2020)
3. http://parts.igem.org/Part:BBa_K880005 (retrieved on 10/10/2020)
4. http://parts.igem.org/Part:BBa_I712074 (retrieved on 10/10/2020)
5. http://parts.igem.org/Part:BBa_B0030 (retrieved on 10/10/2020)
6. https://parts.igem.org/Part:BBa_B0015 (retrieved on 10/10/2020)
7. https://parts.igem.org/Part:BBa_B0016 (retrieved on 10/10/2020)
8. http://parts.igem.org/Part:BBa_K1223006 (retrieved on 10/10/2020)
9. http://parts.igem.org/Part:BBa_K208006 (retrieved on 10/10/2020)
10. http://parts.igem.org/Part:BBa_K864401 (retrieved on 10/10/2020)
11. http://parts.igem.org/Part:BBa_K592012 (retrieved on 10/10/2020)
12. http://parts.igem.org/Part:BBa_K3114005 (retrieved on 25/10/2020)