Team:HK SSC/Basic Part

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BASIC PARTS

Library peptides

BBa_K3561001, BBa_K3561002, BBa_K3561017 and BBa_K3561021 are our library peptides. These peptides have been reported to be able to bind to palladium or reduce palladium from their respective literatures^1,2,3,4.

Single Tryptophan Peptides

BBa_K3561009 to BBa_K3561016 are the peptides with a single tryptophan mutation. As tryptophan residues have been reported to be able to reduce palladium⁴, we modified BBa_K3561002 and replaced different residues with a single tryptophan. The binding residues were not replaced.

Double Tryptophan Peptides

BBa_K3561003 to BBa_K3561008, BBa_K3561019 to BBa_K3561020, BBa_K3561022 to BBa_K3561026 are the peptides with a double tryptophan mutation. As tryptophan residues have been reported to be able to reduce palladium⁴, and a double tryptophan structure have been reported to be more efficient in gold⁵, we replaced different residues with a double tryptophan. The binding residues were not replaced.

Cellulose Binding Domain

BBa_K3561026 is a cellulose binding domain from CipA. The sequence of this cellulose binding domain is provided by Prof. Shen Long Tsai. The related data of this protein has been reported in literature⁶.

Reference

[1] Pacardo, et al. “Biomimetic Synthesis of Pd Nanocatalysts for the Stille Coupling Reaction.” ACS Nano, U.S. National Library of Medicine, 2009, pubmed.ncbi.nlm.nih.gov/19422199/.

[2] Sarikaya, et al. “Molecular Biomimetics: Nanotechnology through Biology.” Nature News, Nature Publishing Group, 2003, www.nature.com/articles/nmat964.

[3] Coppage, et al. “Exploiting Localized Surface Binding Effects to Enhance the Catalytic Reactivity of Peptide-Capped Nanoparticles.” Journal of the American Chemical Society, U.S. National Library of Medicine, 2013, pubmed.ncbi.nlm.nih.gov/23865951/.

[4] Chiu, et al. Size-Controlled Synthesis of Pd Nanocrystals Using a Specific Multifunctional Peptide. 2010, pubmed.ncbi.nlm.nih.gov/20648291/.

[5] DI;, Tan YN;Lee JY;Wang. Uncovering the Design Rules for Peptide Synthesis of Metal Nanoparticles. 2010, pubmed.ncbi.nlm.nih.gov/20355728/.

[6] Yunus, I. S., & Tsai, S. (2015). Designed biomolecule–cellulose complexes for palladium recovery and detoxification. RSC Advances, 5(26), 20276-20282. doi:10.1039/c4ra16200e