Team:HK SSC/Basic Part


BASIC PARTS

Library peptides

BBa_K3561001, BBa_K3561002, BBa_K3561017 and BBa_K3561021 are our library peptides. These peptides have been reported to be able to bind to palladium or reduce palladium from their respective literatures1,2,3,4.

Single Tryptophan Peptides

BBa_K3561009 to BBa_K3561016 are the peptides with a single tryptophan mutation. As tryptophan residues have been reported to be able to reduce palladium4, we modified BBa_K3561002 and replaced different residues with a single tryptophan. The binding residues were not replaced.

Double Tryptophan Peptides

BBa_K3561003 to BBa_K3561008, BBa_K3561019 to BBa_K3561020, BBa_K3561022 to BBa_K3561026 are the peptides with a double tryptophan mutation. As tryptophan residues have been reported to be able to reduce palladium4, and a double tryptophan structure have been reported to be more efficient in gold5, we replaced different residues with a double tryptophan. The binding residues were not replaced.

Cellulose Binding Domain

BBa_K3561026 is a cellulose binding domain from CipA. The sequence of this cellulose binding domain is provided by Prof. Shen Long Tsai. The related data of this protein has been reported in literature6.

Reference

[1] Pacardo, et al. “Biomimetic Synthesis of Pd Nanocatalysts for the Stille Coupling Reaction.” ACS Nano, U.S. National Library of Medicine, 2009, pubmed.ncbi.nlm.nih.gov/19422199/.

[2] Sarikaya, et al. “Molecular Biomimetics: Nanotechnology through Biology.” Nature News, Nature Publishing Group, 2003, www.nature.com/articles/nmat964.

[3] Coppage, et al. “Exploiting Localized Surface Binding Effects to Enhance the Catalytic Reactivity of Peptide-Capped Nanoparticles.” Journal of the American Chemical Society, U.S. National Library of Medicine, 2013, pubmed.ncbi.nlm.nih.gov/23865951/.

[4] Chiu, et al. Size-Controlled Synthesis of Pd Nanocrystals Using a Specific Multifunctional Peptide. 2010, pubmed.ncbi.nlm.nih.gov/20648291/.

[5] DI;, Tan YN;Lee JY;Wang. Uncovering the Design Rules for Peptide Synthesis of Metal Nanoparticles. 2010, pubmed.ncbi.nlm.nih.gov/20355728/.

[6] Yunus, I. S., & Tsai, S. (2015). Designed biomolecule–cellulose complexes for palladium recovery and detoxification. RSC Advances, 5(26), 20276-20282. doi:10.1039/c4ra16200e