Team:HZAU-China/Attributions

Attributions

Project

Project was mainly designed by Yu Han, Shengkun Tong and Yuandong Sui. Other team members also gave advices.

Team training

Experimental training to new team members was conducted by Runzhi Chen, Tianze Meng, Yu Han, Yuandong Sui, Shengkun Tong, and Xinyun Hu.

Modeling training to new team members was conducted by Yun Liu and Zichun Sun.

Safety education was conducted by Professor Yingchun Qi from Huazhong Agricultural University.

Resources

nlmC, nlmAB, comD, comE, lrap, CI, mazF, ptet, pfuA1, and ompA were synthesized by GenScript.

The plasmid contains ClyR, pET-28a(+) vector, and Streptococcus mutans UA159 were provided by Professor Jin He from Huazhong Agricultural University.

The pBAD vector was provided by Professor Donghai Peng from Huazhong Agricultural University.

The pET28a-SUMO vector was provided by Professor Ping Yin from Huazhong Agricultural University.

The positive control of EGFP was provided by Professor Wenyuan Han from Huazhong Agricultural University.

The 3D printer which was used to print hardware “Box” was provided by Professor Bin-Guang Ma from Huazhong Agricultural University.

SnapGene® licenses were generously provided by GSL Biotech LLC.

MATLAB licenses were generously provided by MathWorks®.

Videos

Promotion Video was produced by Yu Han, Shujin She, Yun Liu, Lixiang Xu, Ye Lu, Jianglai Nie, and Mengyao zheng.

Presentation Video is produced by Yu Han, Sheshu Jin, Yun Liu, Lixiang Xu, Ye Lu, Jianglai Nie, Mengyao zheng and other team members.

Art design on the videos is conducted by Lixiang Xu.

Wiki

Codes on our wiki are written by Ao Shen, Xinyun Hu and Heping Zhang.

Art design on the wiki is conducted by Lixiang Xu.

Poster

Poster will be produced by Ao Shen, Yu Han, Yun Liu, Ye Lu, Zichun Sun, Shengkun Tong, and Lixiang Xu.

Art design on the poster will conducted by Lixiang Xu.

Hardware & software

Hardware “Teeth Braces” and “Box” were designed and manufactured by Xuanxuan Huang.

Software “App” was programed by Yu Han and Ao shen.

User interface of “App” was designed by Lixiang Xu.

Experiments

Yu Han and Shengkun Tong took charge in wet lab group and supervised all experiments.

Gene circuits were mainly designed by Yu Han and Shengkun Tong.

Characterization of MazF was designed and performed by Xichen Rao, Xinyun Hu and Ting Xia.

Characterization of PR promoter was designed and performed by Runzhi Chen, and Yue Shu also gave assistance.

Improvement of furA promoter was designed and performed by Shengkun Tong.

Improvement of PRM promoter was designed and performed by Tianze Meng and Ziyun Wu.

Construction and verification of regulatory circuit were conducted by Tianze Meng and Ziyun Wu.

Construction and verification of clyR circuit were conducted by Shengkun Tong and Zhenhao Han.

Construction and verification of phoA circuit were conducted by Shengkun Tong and Zhenhao Han.

Construction and verification of lrap circuit were conducted by Yuandong Sui, and Mengyao Zheng also gave assistance.

Construction and verification of PnlmC and PnlmAB circuits were conducted by Disong Xia, and Zaiyang Chen also gave assistance.

Models

Yun Liu and Zichun Sun took charge in the modeling group and supervised the constructions of all models.

The Detection & report model was constructed by Yun Liu.

The sterilization model was constructed by Yun Liu, Zichun Sun, and Yuanhui Sun.

The Exclusive-OR gate model was constructed by Zichun Sun.

Human Practices

Ye Lu took charge in the HP group and supervised all HP activities.

WeChat tweets were written by HZAU-China.

The typesetting of WeChat tweets was conducted by Jianglai Nie, Mengyao zheng, Shujin She, Ye Lu, and Heping Zhang.

“Love Tooth Encyclopedia” series of popular science videos were produced by Jianglai Nie, Mengyao zheng, Shujin She, Ye Lu, Tianze Meng, and Ziyun Wu.

"Teeth Defense War" event was designed and conducted by Shujin She and Jianglai Nie.

The questionnaires were designed and analyzed by Jianglai Nie, Ye Lu, Ting Xia, and Mengyao zheng.

The online interviews were conducted by Jianglai Nie, Ye lu, and Yu Han.

Daily Management

Runzhi Chen, Ao Shen and Heping Zhang took responsibility for purchasing experimental materials and reagents.

Shengkun Tong took responsibility for the financial affairs on experiment equipments and reagents.

Daily cleaning of our laboratory is assigned to all team members on shift.