Team:Hong Kong CityU/Model
Step 1: Enzymes selection and protein structure modelling
| Enzyme | Substrate (Plastic to be degreaded | Native source of the enzyme |
|---|---|---|
| Poly (ethylene terephthalate) hydrolase (PETase) | Polyethylene terephthalate (PET) | Ideonella sakaiensis strain 201-F6 |
| Papain | Polyurethane (PUR) | Carica papaya |
| Polyurethanase esterase A (PueA) | Polyurethane (PUR) | Pseudomonas Chlororaphis |
| Representing Enzyme | Sequence |
|---|---|
| Polyurethanase esterase A (PueA) | MGVFDYKNFTASDSKALFSDALAITLYSYHNIDNGFAEGYQHNGFGLGLPATLVTALIGSGNSQGVIPG IPWNPDSEKAALDALHQAGWSTISAQQLGYDGKVDGRGTFFGEKAGYGTAQVEILGKYDAQGHLESI GIAFRGTSGPRESVISDTIGDVINDLLAALGPKDYAKNYAGEAFGKLLGDVAAFAQANGLSGKDVLVSG HSLGGLGVNSLADLSSERWSGFYKDSNYIAYASPTQSASDKVLNIGYENDPVFRALDGSTFNLSSLGV HDAHQDSATNNIVNFNDHYASTLWNVLPSSILNIPTWLSHLPTGYGDGLSRVLESKFYDFTSKDSTIV VANLSDPARASTWVQDLNRNAETHKGSTFIIGSDGNDLIQGGSGNDYLEGRAGNDTFRDSGGYNIIL GGQGSNTLDLQQSVKNYSFASDGAGTLYLRDANGGISMTRDIGAIQSKEPGFLWGLFKDDVIHQVTD QGLKAGGQLTQYASSVRGDAGDNVLKAHAGGDWLFGLDGNDHLIGGQGNDVFVGGAGNDLMEA GGGNNTFLFTGHFGQDRILGYQGGDKLVFMGVSGVLPDQDYRAHASSSGNDTVLTFGQDSVTLVGV SLEHLNGSGIVLAMGVFDYKNFTASDSKALFSDALAITLYSYHNIDNGFAEGYQHNGFGLGLPATLVTA LIGSGNSQGVIPGIPWNPDSEKAALDALHQAGWSTISAQQLGYDGKVDGRGTFFGEKAGYGTAQVEI LGKYDAQHLESIGIAFRGTSGPRESVISDTIGDVINDLLAALGPKDYAKNYAGEAFGKLLGDVAAFAQA NGLSGKDVLVSGHSLGGLGVNSLADLSSERWSGFYKDSNYIAYASPTQSASDKVLNIGYENDPVFRA LDGSTFNLSSLGVHDAHQDSATNNIVNFNDHYASTLWNVLPSSILNIPTWLSHLPTGYGDGLSRVLE SKFYDFTSKDSTIVVANLSDPARASTWVQDLNRNAETHKGSTFIIGSDGNDLIQGGSGNDYLEGRAG NDTFRDSGGYNIILGGQGSNTLDLQQSVKNYSFASDGAGTLYLRDANGGISMTRDIGAIQSKEPGFL WGLFKDDVIHQVTDQGLKAGGQLTQYASSVRGDAGDNVLKAHAGGDWLFGLDGNDHLIGGQGN DVFVGGAGNDLMEAGGGNNTFLFTGHFGQDRILGYQGGDKLVFMGVSGVLPDQDYRAHASSSGN DTVLTFGQDSVTLVGVSLEHLNGSGIVLA |
Mathematical models and computer simulations provide a great way to describe the function and operation of Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab.
Gold Medal Criterion #3
Use modeling to gain insight into how your project works or should be implemented. Explain your model's assumptions, data, parameters, and results in a way that anyone could understand.
Please see the 2020 Medals Page for more information.
Best Model Special Prize
To compete for the Best Model prize, please describe your work on this page and also fill out the description on the judging form. Please note you can compete for both the Gold Medal criterion #3 and the Best Model prize with this page.
Inspiration
Here are a few examples from previous teams: