iGEM Lund 2019

Parts

Our parts consist of basic parts coding for fusion partners, protease, protease cleavage site, 6xHis-tag and our set of eight AMPs. The composite parts are built up by these basic parts into two major sets: AMPs fused to partner Thioredoxin 1 and AMPs fused to partner Glutathione-S-Transferase. We have a separate composite part for expression of the protease Enterokinase, which uses the fusion partner Thioredoxin 1. There is also a Thioredoxin control part without the AMP region.

Basic Parts

TrxA: BBa_K3619001

Encoding the protein Thioredoxin 1 which was used as a fusion partner.

GST: BBa_K3619002

Encoding the protein Glutathione S-Transferase which was used as a fusion partner.

Enterokinase: BBa_K3619003

Encoding the protease Enterokinase, which would be used to cleave the fusion proteins.

Enterokinase cleavage site: BBa_K3619004

Encoding the cleavage site of Enterokinase, added between fusion partner and AMP.

6xHis tag: BBa_K112703

Encoding a his-affinity tag for possible later purification.

Anti-microbial peptides

Ap24: BBa_K3619005

EAFP1: BBa_K3619006

Ec-AMP-D1: BBa_K3619007

MsrA2: BBa_K3619008

Rs-AFP1: BBa_K3619009

nmDEF02: BBa_K3619010

Scarabaecin: BBa_K3619011

Temporin A: BBa_K3619012

Composite Parts

Protease

Trx-Ek: BBa_K3619030

AMP parts

Trx-Ap24: BBa_K3619013

Trx-EAFP1: BBa_K3619014

Trx-Ec-AMP-D1: BBa_K3619015

Trx-MsrA2: BBa_K3619016

Trx-Rs-AFP1: BBa_K3619017

Trx-nmDEF02: BBa_K3619018

Trx-Scarabaecin: BBa_K3619019

Trx-Temporin A: BBa_K3619020

GST-Ap24: BBa_K3619021

GST-EAPF1: BBa_K3619022

GST-Ec-AMP-D1: BBa_K3619023

GST-MsrA2: BBa_K3619024

GST-nmDEF02: BBa_K3619025

GST-Rs-AFP1: BBa_K3619026

GST-Scarabaecin: BBa_K3619028

GST-Temporin A: BBa_K3619029

Control

Thioredoxin: BBa_K3619031