Team:NAU-CHINA/Notebook

March

  • Set up NAU-CHINA 2020 team!

April

  • Brainstormed and researched literature, found several directions which we were interested and conceived our projects.
  • Consulted relevant experts and selected the feasibility of our projects.

May

  • Determined the subject--SLIM and investigated the background of the subject.
  • Determined the Bacillus subtilis as engineered bacteria and earthworms as carrier.
  • Registered our team and determined our team members.
  • Exchanged with Nanjing University for the first time to discuss HP's stakeholders.
  • Opening festival.
  • Built earthworms immobilizing lead model.

June

  • Determined functional elements, regulatory elements, and anaerobic regulatory elements and built gene circuit.
  • Meetup of South China.
  • Constructed the framework of wiki.
  • Cooperated with One Health International Association.
  • Interviewed experts on lead poisoning and soil environment.

July

  • Optimized the design of gene circuit.
  • Designed experimental schemes and expected results.
  • Learnt to use SnapGene and used SnapGene to construct RFC10 standard plasmid.
  • Constructed the framework of wiki.
  • Interviewed agricultural economic experts.
  • Interviewed the stakeholders.

August

  • Attended the 7th CCiC.
  • Reconstructed gene circuit, added the kill switch and replaced functional elements with phytase (ycD).
  • Completed safety form & check in form.
  • Global meet up!
  • Edited wiki.
  • Conducted science communication with Nanjing-China.

September

  • Redesigned the experimental scheme and added kill switch.
  • Made promotion video.
  • Held iGEM campus promotion (on the Yulan Road).
  • Edited wiki.
  • Completed the picture book The Adventure of Andrew.
  • Completed music creation.

October

  • Verified the overall gene circuit, simulation results.
  • Built two mathematical models: CA model and kill switch model.
  • Edited wiki.
  • Drew Poster.
  • Prepared for presentation video.
  • Held iGEM campus promotional activities.
  • Celebrated wiki freeze!