Team:NJMU-China/Contribution
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Overview of Contributions
As for existing parts, we mainly added knowledge to previous biosensors concerning lasR/lasI signaling system, which were originally used to sense Quorum-Sensing molecules like HSL etc.
As for new parts, we designed and constructed a novel serotonin biosensor based on lasR/lasI signaling system. We characterized it with modeling of the interactions. We also attempted a design of positive feedback in cope with low transcriptional activity of the induced promoter PlasI.
Existing Part We Contributed
Through massive literature investigation, we found that serotonin acts as an interkingdom signaling molecule via quorum sensing. Specifically, no response was noted in the rhl cellular system, indicating specificity for the las QS pathway.
We evaluated the docking scores between the complex of LasR (a signaling molecule in las QS pathway) - Serotonin/HSL (QS molecule in las QS pathway) and PlasI, and found that serotonin has similar interaction patterns with HSL in compared with negative control. Therefore, we reasonably consider it is appropriate to add this information to existing lasR/PlasI related parts (e.g. BBa_K726001).
To learn more, you could see BBa_K726001.
New Parts Submitted to the Registry
Based on this knowledge mentioned above, we designed, constructed and modified 3 parts:
| Name | Structure | Function |
|---|---|---|
| BBa_K3631000 | Ptac-lasR (Coding) | Express LasR Proteins |
| BBa_K3631100 | PlasI-lasI (Coding)-lacZ (Coding) | Visible Reporter System with Improved Induction Efficacy |
| BBa_K3631300 | BBa_K3631000 & BBa_K3631100 | Serotonin Whole Cell Biosensor |
To learn more, you could see Resultsand Simulations of Interactions.