Team:OSA/JUDGING Medal

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• PROJECT
  • Description
  • DNAzyme
  • Signal Amplification System
  • DNAzyme Mutation
  • Proof of Concept
  • Model
  • Engineering Success
• Human Practice
  • Integrated Human Practice
  • Public Engagement
  • Proposed Implementation
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CRITERIA

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• 1. Competition deliverables

  2. Attribution

  3. Project Description

  4. Contribution

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• We have submitted all competition deliverables

  Attributions in Wiki Page

  Project-Introduction in Wiki Page

  Judging in Wiki Page
  We designed and tested the 8-17 DNAzyme, while also mutating the gene to create ore efficient methods throughout multiple trials. We succeeded in the end in which a significanctly more efficient sequence is found. We consider this as a unique contribution. Previous teams(like Team Pittsburgh from 2016) have attemtped to conduct similar trials but failed. However, we achieved a positive result.

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• 1. Engineering Success

  2. Collaboration

  3. Human Practices

  4. Proposed Implementation

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• Engineering in Wiki Page
  We combined our testing scenario and after many rounds of eliminating, acquired an usable signal amplification system for our project.

  Collaboration in Wiki Page
  We shared laboratories with other Shanghai teams and participated in multiple meetups in the iGEM community.

  Human Practices in Wiki Page
  We interviewed a wide variety of industries and implemented the opinions of experts into our second phase of experiment, in which we made innovations like the test strip.

  Implementation in Wiki Page
  We have conducted many investigations on the application scenarios of DNAzyme testing products. After the investigation, we made a clear plan for product application scenarios. For the two different use scenarios of water and soil, we have made different attempts in the laboratory.

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• 1. Integrated Human Practices

  2. Project Modeling

  3. Proof of Concept

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• IHP in Wiki Page
  Through interviewing several industries, we learned that this product is more suitable for the testing of soil heavy metal ions. Shifting our focus, we conducted this experiement for heavy metal ions in soil in the laboratory as a preliminary attempt.

  Model in Wiki Page
  For the Cas12a reaction process, we analyzed and simulated it using Mie equation. Since we can use ssDNA fragments of different lengths as input signals, we need to know which length of signal is most suitable. Therefore, we use the Mie equation to describe this combination process, which provides convenience for future use.

  Proof of Concept in Wiki Pag
  We successfully detected heavy metal ions in an unknown solution using the DNAzyme signal amplification system and was able to infer its the concentration.