Team:Pittsburgh/Contribution

Bluetooth Bacteria

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Contribution

 

Temperature Sensitive Plasmids & Magnetic Nanoparticles

We characterized the temperature-sensitive plasmids pTlpA-Wasabi, pTlpA-Wasabi-NF, pDnaK-Wasabi, pRpoH-Wasabi, pLaqI-Wasabi, pClp-Wasabi​, PHtpG-Wasabi, and PGrpE-Wasabi​ from Mikhail Shapiro's lab. Each plasmid contains a transcriptional regulator along with the sequence encoding for Wasabi, a green fluorescent protein. pTlpA-Wasabi-NF encodes for a non-fluorescent version of Wasabi. We characterized the expression of these constructs in E. coli BL21 by inducing with varying temperatures in a PCR machine and monitored OD600 and fluorescence over different hours.

In addition, we also created a protocolin which future teams can use to conjugate amine-functionalized magnetic nanoparticles to the surface of bacteria.