Team:UM Macau/Parts
Part Collection
Basic Parts
| Part Number | Name | Type | Description | Length(bp) |
|---|---|---|---|---|
| BBa_K3630000 | T7 promoter | Regulatory | The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase. The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications. | 19 |
| BBa_K3630001 | Lac operator | Regulatory | The lactose operon (Lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria. | 25 |
| BBa_K3033002 | RBS | RBS | A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA transcript that is responsible for the recruitment of a ribosome during the initiation of protein translation. | 6 |
| BBa_K3630003 | LuxR | Coding | LuxR is a constitutively expressed protein that can bind with AHL. | 750 |
| BBa_K3630004 | S-tag | Tag | The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. | 45 |
| BBa_K3630005 | LuxR promoter | Regulatory | LuxR promoter controls transcription of the LuxI enzyme leading to a positive feedback loop that increases transcription from the right hand lux promoter. | 55 |
| BBa_K3630006 | His-tag | Tag | A His-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. | 18 |
| BBa_K3630007 | DspB | Coding | DspB catalyzes the hydrolysis of linear polymers of N-acetyl-D-glucosamines found in the biofilm matrices and allows for the release of bacterial cells that can adhere to new surfaces close by and extend the biofilm or start new colonies. | 1086 |
| BBa_K3630008 | DNaseTA | Coding | A deoxyribonuclease (DNase, for short) is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA. | 1083 |
| BBa_K3630009 | Alkaline serine protease | Coding | Serine proteases (or serine endopeptidases) are enzymes that cleave peptide bonds in proteins, in which serine serves as the nucleophilic amino acid at the (enzyme's) active site. | 444 |
| BBa_K3630010 | Adhesin | Coding | Proteins on the surface of living cells that allow the organism to colonize various surfaces. | 3117 |
| BBa_K3630011 | GFP | Coding | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. | 717 |
| BBa_K3630013 | Ho1 | Coding | Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake. | 867 |
| BBa_K3630014 | PcyA | Coding | Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. | 738 |
| BBa_K3630015 | OmpC promoter | Regulatory | Forms pores that allow passive diffusion of small molecules across the outer membrane. | 123 |
| BBa_K3630016 | Lac repressor | Coding | Lacl is a DNA binding protein that inhibits the expression of lactose coding gene metabolism in bacteria. When lactose is not stimulated, lacl will be suppressed. When lactose is available, it will be converted to allolactose, by the Lac repressor. | 1113 |
| BBa_K3630017 | iBox | Coding | An inhibitor of protein kinase PAK4. It contains two copies of the kinase inhibitory domain. These small regions supports PAK4cat crystal formation in cells. When inka-box forms a complex with PAK4cat, conformational changes causes the complex to spontaneously crystallize and produce long rod-shaped crystals. | 38 |
| BBa_K3630018 | PAK4 cat | Coding | PAK4 is a kinase that is linked with –inka-Box, which has strong links to cellular transformation. Together they form crystallized protein structures. This crystallization provides more storage space for iron. | 314 |
| BBa_K3630019 | HlyA | Coding | E. coli α-hemolysin (HlyA) produces large, clear zones of hemolysis around colonies on blood agar. HlyA lyses cells by the creation of pores in the target cell membrane and affects erythrocytes, leukocytes nd renal tubular cells . Its activity on polymorphonuclear granulocytes liberates leukotrienes, histamine, and ATP and is neutralized by specific antiserum. | 314 |
Composite Parts
| Part Number | Name | Component Parts | Description | Length(bp) |
|---|---|---|---|---|
| BBa_K3630020 | AHL dependent enzyme expression system-DspB | BBa_K3630001, BBa_K3630002, BBa_K3630003, BBa_B0010 | AHL binds to LuxR and then LuxR binds to LuxR promoter to promote DspB to degrade the biofilm. | 2231 |
| BBa_K3630021 | AHL dependent enzyme expression system-DNaseTA | BBa_K3630005, BBa_K3630002, BBa_K3630006, BBa_K3630008, BBa_K3630019, BBa_K3630000, BBa_K3630001, BBa_K3630002, BBa_K3630003, BBa_B0010 | AHL binds to LuxR and then LuxR binds to LuxR promoter to promote DNaseTA to degrade the biofilm. | 2228 |
| BBa_K3630022 | AHL dependent enzyme expression system-alkaline serine protease | BBa_K3630005, BBa_K3630002, BBa_K3630006, BBa_K3630009, BBa_K3630019, BBa_K3630000, BBa_K3630001, BBa_K3630002, BBa_K3630003, BBa_B0010 | AHL binds to LuxR and then LuxR binds to LuxR promoter to promote alkaline serine protease to degrade the biofilm. | 1589 |
| BBa_K3630023 | AHL dependent adhesive protein-GFP expression | BBa_B0010, BBa_K3630000, BBa_K3630001, BBa_K3630002, BBa_K3630003, BBa_B0010 | AHL binds to LuxR and then LuxR binds to LuxR promoter to promote adhesive protein to attach the biofilm. | 4855 |
| BBa_K3630025 | PCB formation LuxR-OmpC dependent expression system | BBa_J23107, BBa_K3630002, BBa_K3630013, BBa_K3630002, BBa_K3630014, BBa_B0010, BBa_J23107, BBa_K3630002, BBa_I15010, BBa_B0010 | Constitutively promote PCB , which requires Ho1 and PcyA and constitutively promote Cph8 receptor. | 4091 |
| BBa_K3630027 | Constitutive LuxR expression and dark repression system | BBa_J23107, BBa_K3630001, BBa_K3630002, BBa_K3630003, BBa_B0010, BBa_K3630015,BBa_K3630016, BBa_B0010 | Constitutively expresses LuxR that has an expression regulated by the lac operon. Conditionally expresses LacI gene under the ompC promoter. | 2212 |
| BBa_K3630029 | Enhanced ferritin expression system | BBa_J23106, BBa_K3630002, BBa_K3033014, BBa_K3630017, BBa_K3630018, BBa_B0010 | Constitutively promotes ferritin expression. The ferritin has been linked to iBox-PAK4 which forms crystal structures and enhances the bacteria's sensitivity to magnetic force. | 1004 |