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Attributions

Index

Team Attributions
Acknowledgements

Team Attributions

The whole team has been actively participating in brainstorming, researching, planning and presenting the project. Below is a compilation of individual efforts.

Name and Title	Involvement
Anisha Khan: Team's doing things properly spirit	Graphical Design group leader, part of the group working on DBD system. Extra effort put into part and primer design, the proof of concept caffeine assays and documentation of experiments. Special effort in team hoodies design, biosafety and promo video filming, creating illustrations for the Design page on Wiki and managing team's social media.
Linnéa Yuan Andersson: Team's TikTok master	Part of Graphical Design with special effort in logo design and part of the group working with DBD system. Vital role in bacterial culturing, PCRs and electrophoresis. Special effort in editing the biosafety videos, taking as well as editing the individual and group pictures of the team and managing team's TikTok account.
Björn Ancker Persson: Team's laser focus	Wiki group leader and part of the lab group working with Type IIS assembly. Special effort in cloning the optimized system elements.
Milica Plavsic: Team's deep learner	Part of the Modelling group and lab group working on NarX-NarL. Extra effort put into Bacillus experiments i.e cloning and optimization of the protocols.
Elnaz Samandianzad: Team's most curious person	Part of the Human Practices and lab group working on DBD system. Special effort in part and primer design, bacterial culturing and transformation, optimizing the spheroplast experiments, outreach in education purposes and preparing the final presentation.
Samuel Zargani: Team's fast learner	Part of the Wiki and lab group working on reporter system and agglutination approach. Special effort in the agglutination approach, researching alternatives for reporter system and cloning experiments.
Rubén Crespo Blanco: Team's multitasker	Part of the Human Practices and of lab group working on DBD system. Special effort in data analysis of the caffeine assays with DBD system, planning and carrying through experiments. Further effort put in agglutination construct design, prototype design, lyophilization experiments, team presentations during summer, advising the whole team in wet lab and writing the Implementation page on the Wiki.
Alexandra-Livia Vilcu: Team's steady hand for camera and pippetes	Part of the Graphical Design and lab group working on reporter system. Special effort in supporting the lab group, beta-galactosidase transformation and enzyme activity measurements, team's GoFundMe video, postcards, photographs for the team members, filming for biosafety videos and promo video, design and edit icons for the project. Part of the final presentation video.
Pedro Nunes: Team's ambitious spirit	Part of Sponsoring and leader of the lab group working on the NarX-NarL system in B. subtilis. Extra effort put into planning and carrying through B. subtilis experiments i.e. sequence design, cloning and optimization of the protocols; project development, especially in the early stages; compilation of the contributions page; collaboration with Team Generation Mendel from Brno University.
Dorottya Marko: Team's realist	Part of Graphical Design and lab group working with Type IIS assembly. Special effort in preparing Type IIS cloned parts for sequencing and analyzing the results, creating the main overview picture explaining project design and illustrations for the Type IIS guide book.
Sebastian Moes: Team's Modelling master	Leader of Modelling and part of the lab group working on the reporter system and agglutination approach. Special effort in teaching efforts for the modelling group, developing and carrying through the dry lab experiments. Team presenation during summer and final presentation.
Saria Azam: Team's leap from computations in biology	Part of the Modelling group. Extra effort put into selection of docking poses. Helped during creation and running of the MD simulation pipeline.
Nicole Eger: Team's pushing through setbacks	Leader of the lab group working on the reporter system and agglutination approach, part of the Modelling group. Special effort in planning and carrying through groups experiments, advising the whole team in wet-lab. Preparing the final presentation.
Therese Koivula: Team's respecting deadlines	Leader of the Human Practices and co-leader of the lab group working on the NarX-NarL system. Special effort in part design and cloning of the NarX system, From paper to reality event organization, organizing outreach contacts for Tuberculosis research and education purposes.
Thomas Andersson: Team's performer	Part of the Human Practices and lab group working on the reporter system and the agglutination approach. Special effort in the agglutination approach, organizing material from interviews and biosafety videos.
Tereza Hubáčková: Team's finding solutions	Leader of the lab group working on the Type IIS assembly and part of Human Practices. Special effort in planning and carrying through groups experiments, data analysis of caffeine assays with DBD system, advising the whole team in wet lab and project development, planning of the Type IIS guidebook. Wiki layout planning.
Hui Yu: Team's eternal good mood	Part of Modelling and lab group working with Type IIS assembly. Special effort in cloning the optimized system elements.
Martin Gonzalez Burgos: Team's natural leader	Leader of the lab group working with DBD-mRFP1 system and part of the Wiki group. Special effort in planning and carrying through groups experiments, advising the whole team in wet lab and designing the Meet The Team page on Wiki. Team presentations during summer and the final presentation.
Louise Castro de Jesus: Team spirit 10 000 km apart	Part of the Sponsoring and lab group working on the reporter system and agglutination approach. Special effort in Human Practice with interviews in Brazil and in Graphical Design with logo development, postcard design, editing of the promo video and front page of the Wiki.
Emelie Bringeland: Team's eye for details	Leader of Sponsoring and co-leader of lab group working on NarX-NarL system. Special effort in organizing safe lab environment, part and primer design, cloning of NarX system, TB pamphlet and tuberculosis research, From Paper To Reality event, managing team's social media pages. Organizer of team-building weekend.
Núria Garriga Alonso: Team's has all of the answers	Co-leader of Wiki and part of the lab group working with Type IIS assembly. Special effort in planning and carrying through groups experiments, advising the whole team in wet lab, development of the Type IIS guidebook, planning and coding Wiki layout.
Xiguo He: Team's prototyping expert	Part of the Modelling group. Stochastic modelling. Special effort in developing the 3D models of the prototype.
Israa Aly Abdou: Team's getting tasks done	Part of Sponsoring and lab group working on the reporter system and agglutination approach. Special effort in beta-galactosidase transformations and enzyme activity measurements, Human Practices interviews, team hoodie design.
Blanca Valero Martínez: Team's adapt and overcome anything	Project manager. Special effort in recruiting the team, organizing workshops, Safety form, effective task planning and managing, planning adaptations to Covid-19 restrictions, administrative tasks, graphical design, Wiki development , project promo and presentation videos.
Ruta Upite: The soul of the project	Project manager. Special effort in recruiting the team, organizing workshops, Safety form, effective task planning and managing, planning adaptations to Covid-19 restrictions, administrative tasks, accounting. Head of Wiki concept development and Human Practices presentations.

Margareta Krabbe

As our primary PI, Margareta Krabbe has helped us from the early stages of the team recruitment to the very end. With her help it has been possible to tackle the administrative questions of lab space, adaptations to Covid19 restrictions and safety education. She has also given valuable input on the project itself. Her tireless work for active education and recognition of iGEM as a place for it has enabled our teams participation in the competition.

Anthony Forster

Our secondary PI, professor Forster has helped us throughout the whole project. From the early stages we have received valuable feedback allowing us to research gaps in our project design, troubleshoot our experiments and present science in a proper way. His dedication to science has helped us and our project become better.

Emil Marklund

Our instructor, Emil Marklund has been the greatest support we could have dreamt of. Emil has been there for all of our questions from our first steps in BioBrick design, through failed clonings, all the way to the assays for proof of concept. He has been explaining and teaching at times when we were in over our heads, celebrating and reviewing interpretations of our triumphs. His ability to perceive our goals, our backgrounds and science in general has helped us to conduct our project.

Acknowledgements

Here we have gathered all of the external supporters of team Uppsala 2020. Without your efforts our project would not have come this far. Thank you!

General Support

Biology Education Centre (UU)

For providing our team with a laboratory and equipment. Our team could never have done the work we did without this critical support. Special thanks to the head of the department, Henning Blom in being open for discussions on teams needs and work in the shadow of pandemic restrictions.

iGEM Uppsala Association

For connecting us with previous iGEM Uppsala teams and providing valuable advice.

Peter Lillhager (UU)

For instructing us in general safety and helping us with ordering and receiving materials. Peter’s support helped our working environment greatly.

Karina Lagerström (UU)

For instructing us in general safety and on how to keep the lab clean . Karina's support helped us orientate in the laboratory from early on.

Banafsheh Seyyed Khezri (UU)

For instructing us in general safety and helping us to locate needed equipment and chemicals.

Mats Omarin (UU)

For providing us with lab equipment and helping us with anything we needed around in the lab.

Mikael Olsson (UU)

For educating us on safety and waste handling routines at the Biomedical Centre at Uppsala university.

Henrik Gradstedt (UU)

For instructing us in general biosafety and holding a tutorial on spillage handling.

Research Support

Isabel Sá-Nogueira

Head of the Microbial Genetics Laboratory - UCIBIO (FCT-UNL)
For providing the B. subtilis natural competence protocol

Gunnar Johansson (UU)

Professor at Department of Chemistry - BMC, Biochemistry
For advice in protein purification of HSP16.3 protein and providing us with the materials of the purification.

Daniel R. Zeigler

Director of Bacillus Genetic Stock Center
For providing KO7 strain and vectors for B. subtilis transformation (pBS1C and pBS2E) and being available to answer questions about B. subtilis.

Sanna Koskiniemi (UU)

Senior lecturer/Associate Professor at the Department of Cell and Molecular Biology, Microbiology.
For advice during idea development and on how to perform agglutination related experiments.

Letian Bao (UU)

M.Sc. Student in professor Forsters lab
For his advice, recommendations and suggestions on our experimental design.

Yannick Haye (UU)

M.Sc Student, iGEM Uppsala alumni (2019)
For advising the team in protein purification protocols and instructing the team in the wet lab for these experiments.

Jonas Gockel

PhD Student at Radboud University, iGEM Uppsala alumni (2019).
For advice for the team regarding work with B.subtilis as a model organism and Gibson Assembly.

Daniel Camsund (UU)

Post-doctoral researcher at the Department of Cell and Molecular Biology, Molecular Systems Biology
For advice for the team regarding cloning and testing the constructs .

Jakub Wiktor (UU)

Post-doctoral researcher at the Department of Cell and Molecular Biology, Molecular Systems Biology
For advice during idea development.

Modelling Support

Florian van der Ent (UU)

PhD student at Department of Cell and Molecular Biology, Computational Biology and Bioinformatics
Assisted in development of the modelling strategy for the molecular dynamics approach and advice.

Tobias Jakobsson (UU)

Project Manager at Biology Education Centre
Assisted in configuration of hardware and software for modelling subgroup.

Katherin Vallejos

iGEM Uppsala alumni (2019)
Advice and help with modelling, especially molecular dynamics

Graphical Design Support

Andreas Myrén

For helping us film drone videos for our promotional video.

Mark Harris

Photographer
For advising the graphical design group on filming equipment.

Amy Andersson

Graphic designer
For helping us in the design of our logo.

Vecteezy

https://www.vecteezy.com/
For open source graphics e.g. social media icons used in our wiki.

Freepik

https://www.freepik.com/
For open source graphics e.g. infographics used in our wiki.

Flaticon

Smashicons from www.flaticon.com

https://www.flaticon.com//
For open source vector graphics e.g. social media icons used in our wiki

Wiki Support

Mariam J.Ndwatta

M.E. Student, iGEM Uppsala alumni (2019)
For advising and helping the non-computational science wiki group greatly

Human Practices Support

Nuno Ramos Rufino de Sousa

Laboratory technician at Karolinska Institutet
For participating in an interview with the HP team to answer questions about diagnosing tuberculosis and how we can improve our kit.

Leif Kirsebom (UU)

Professor at Department of Cell and Molecular Biology
For participating in an interview with the HP team to answer questions about diagnosing tuberculosis and how we can improve our kit. He also provided us with a whole beta-galactosidase on a plasmid vector and presented us with the possibility to test our HSP nanobodies against HSP in mycobacterium marinum and that his lab also has access to the BCG bovis strain.

Marlucia Garrido

State coordinator of the Tuberculosis Control Program in Amazonas
For participating in an interview to answer questions about diagnosing tuberculosis and how we can improve our kit.

Lars Hellman (UU)

Professor at Department of Cell and Molecular Biology
For participating in an interview with the HP team to answer questions about diagnosing tuberculosis and how we can improve our kit.

Kristian Jelse

Environmental consultant at Greendesk
For Life Cycle Assessment lecture.

Charlie Carpene

Freelancing prototyping consultant
For giving us valuable feedback on the design of our prototype-

Adam Engberg (UU)

Research Engineer at U-PRINT, 3D-printing facility
For introduction in 3D printing and printing our prototype. Adams' support helped us visualize and evaluate our work.

Olle Pontén

M.E. Student, iGEM Uppsala alumni (2016)
For advising us in Fusion 360 software while designing our prototype.

FIND

The Foundation for Innovative New Diagnostics
For consulting us on current tuberculosis detection tools and targets.

Team:UofUppsala/Attributions