Our products - background
We will introduce a production pathway for salicylic acid in Synechocystis and E.coli. The main reason for choosing salicylic acid as a target compound is because it requires a pyruvate dependent strain. The construction of such a strain allows for the rapid implementation of many other Forbidden FRUITS strategies for many other different products. This is because pyruvate can be seen as a central metabolic hub. And therefore, it is relatively easy to find reactions that link any given compound to it. This illustrates that a single Forbidden FRUITS strategy can be used to develop a chassis for the production of a variety of compounds.
Salicylic acid is the benzoic precursor of the painkiller aspirin. Besides being used in the pharmaceutical industry, it is also applied in food preservation and cosmetics. Until present it is chemically synthesized using the Kolbe-Smith reaction (Brown, 2016). Naturally occurring in plants, an metabolic pathway to produce salicylic acid is readily available. Therefore, this compound has the potential of being produced by microbial systems.
Lactate serves as a resource for the production of bioplastics. Besides having great potential in the production of a biodegradable plastic, it can also be used in food, cosmetics and pharmaceuticals. Lactate can be produced by a chemical or fermentation process. Because of the increasing demand for lactate in its many applications, it is important to make the production process more efficient and sustainable (Abdel-Rahman, et al. 2011).
Mannitol is a sugar with application in the food, pharmaceutical and chemical industry. It can be produced from plant
material or in a chemical process. Because of the environmental impact and the costs of both methods, the demand
for a different production process is increasing (Madsen, et al. 2018). By implementing a mannitol production
pathway, we show the possibility of producing this compound in a sustainable and cost effective manner. The
mannitol production pathway is an example of a path optimized using the cheap lunch strategy. For more
information about Cheap Lunch strategies, please visit our results page.
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