Team:CSMU Taiwan/Notebook
05/20 Transformation, Toehold Test
05/21 Agar plate preparation, Culturing bacteria, toehold test
05/22 Plasmid extraction, RE check
05/25 toehold test
05/27 toehold test
05/14 Transformation
05/18 Culturing bacteria
05/19 Bacteria storage, Plasmid Extraction, RE check
05/20 Transformation, Toehold Test
05/21 Agar plate preparation, Culturing bacteria, toehold test
05/22 Plasmid extraction, RE check
05/25 Culturing Bacteria
05/27 Plasmid Extraction
05/28 Transformation
05/29 Culturing bacteria
05/30 Plasmid Extraction, RE Check
05/14 Transformation
05/18 Culturing bacteria
05/19 Bacteria storage, Plasmid Extraction, RE check
05/20 Transformation, Toehold Test
05/21 Agar plate preparation, Culturing bacteria, toehold test 05/22 Plasmid extraction, RE check
05/25 toehold test, Culturing Bacteria
05/26 Plasmid Extraction, RE check
06/04 Toehold Test
06/01 Gel Extraction, Clean up, Electrophoresis, Ligation,
06/24 PCR, PCR Clean Up
06/29 PCR, PCR Clean Up
06/30 Toehold Test, mRFP Test, PCR, PCR Clean Up
06/02 Culturing Bacteria
06/03 Bacterial Storage
06/23 Culturing Bacteria
06/30 Toehold Test, mRFP Test, PCR, PCR Clean Up
07/01 Toehold Test, Invertase test, mRFP pH Test
07/03 PCR, Invertase test, Toehold Test, mRFP pH Test
07/10 Invertase test, Toehold Test
07/13 Invertase Test, PCR
07/14 Invertase test, Plasmid Extraction
07/02 Toehold test, mRFP pH Test
07/09 Toehold test
07/10 Invertase test, Toehold Test
07/13 Invertase Test, PCR
07/17 PCR
07/19 PCR
07/20 Resuspension
07/21 Gel Preparation, Transformation
07/22 Culturing bacteria
07/23 Plasmid extraction, RE check
07/24 Ligation, Transformation
07/27 Transformation, Culturing Bacteria
07/28 Bacteria Storage, Plasmid Extraction, Culturing Bacteria
07/29 Bacteria Storage, Plasmid Extraction
07/31 PCR, Clean Up
08/03 Clean Up, Ligation, Transformation
08/04 Colony PCR, Culturing Bacteria
08/05 Bacteria Storage, Plasmid Extraction, RE check, RE for Ligation
08/06 Clean Up, Ligation
08/07 Culturing
Bacteria
08/08 Plasmid Extraction, RE check
08/20 Resuspension, Transformation
08/21 Culturing Bacteria
08/22 Bacteria Storage, Plasmid Extraction
08/24 AmilCP Test
08/25 AmilCP Test
08/26 AmilCP Test, Culturing Bacteria
08/27
Plasmid Extraction, AmilCP Test
9/4 Toehold test, transformation
9/5 Toehold switch transformation
9/6 Culture bacteria
9/7 Plasmid extraction, Glucose concentration detection
9/8 Plasmid extraction
9/9 Culture bacteria
9/10 Plasmid extraction, Glucose concentration detection
9/12 Bacteria storage,Plasmid extraction, RE check, gel electrophoresis
9/19 Sensitivity test
9/22 Specificity test
9/26 "ZR" toehold test with different amount of trigger
9/27 "ZRA" toehold test with different amount of trigger