Team:DeNovocastrians/Parts
BBa_K3694023
The benABCD gene clusters isolated from Rhodococcus strain 9, is the benzene degradation pathway degrading benzene into catechol. The cluster encodes for a benzoate 1,2-dioxygenase (benABC) which converts benzene into Cis-1,2-dihydroxycyclohexadiene. This is then converted to catechol by the benD encoded dehydrogenase.
BBa_K3694024
The benABCD gene cluster isolated from ADP1,which encodes for the benzene degradation pathway that degrades benzene into catechol. The cluster encodes for a benzoate 1,2-dioxygenase (benABC) which converts benzene into Cis-1,2-dihydroxycyclohexadiene. This is converted to catecol by the benD encoded dehydrogenase.
Biosensor
BBa_K3694026
Biosensor gene construct that would work in E.coli. The construct contains transcription factor CaTM and its binding site connected to a downstream fluorescent mCherry gene. The transcription factor, in the presence of its ligand catechol or cis,cis-Muconate, would bind its binding region to recruit RNAP and transcribe the downstream fluorescent protein gene. The construct also contains benM transcription factor and its binding region which is connected to a downstream green fluorescence gene. In the presence of benzene and cis,cis-Muconate, the transcription factor binds to the binding region to recruit RNAP and transcribe the downstream fluorescent protein gene
benE
BBa_K3694027
benE gene obtained from ADP1 genomic DNA - encodes for a transmembrane symporter which enables cells to import benzene/benzoate from their environment.
BBa_K3694028
benE gene obtained from synthesized E. fergusonii DNA - encodes for a transmembrane symporter which enables cells to import benzene/benzoate from their environment.
BBa_K3694029
benE gene obtained from Rhodococcus strain 9 genomic DNA - encodes for a transmembrane symporter which enables cells to import benzene/benzoate from their environment.
Parts List
| Name | Type | Description |
|---|---|---|
| BBa_K3694023 | Coding | benABCD gene clusters isolated from Rhodococcus strain 9 |
| BBa_K3694024 | Coding | benABCD gene cluster isolated from ADP1 |
| BBa_K3694026 | Device | Biosensor |
| BBa_K3694027 | Transporter | benE gene obtained from ADP1 |
| BBa_K3694028 | Transporter | benE gene obtained from synthesized E. fergusonii |
| BBa_K3694029 | Transporter | benE gene obtained from Rhodococcus strain 9 |