Team:GDSYZX/Collaborations

• Collaborations

COLLABORATIONS

In the progress of perfecting our project, we had collaborations with other teams, include BNDS_China from Beijing, SYSU-CHINA from Sun Yat-sen University and SJTU-BioX-Shanghai from Shanghai Jiao Tong University.

During the exchange with BNDS_China, our team made a speech about our team project, introducing the background of the project, the idea of designing the experiment, the results of the final experiment and HP for them. BNDS_China introduced us to their design ideas and objectives of their project: to make environmentally friendly artificial leather with customizable printing based on bacterial cellulose and protein modification. In addition, they introduced the principles of their experiments and the hardware products under development. By applying bacterial cellulose skeleton and bacterial cellulose skeleton as well as protein cross-linking technology, their imagined finished products will have many useful characters, such as toughness, wear resistance, softness, elasticity, high structural strength, etc.

At the end of the communication, Li Wanji and Lin Lihan of BNDS_China raised questions about our project and put forward constructive suggestions for it, including opinions on the determination of HQT gene expression: can the expression of green fluorescent protein replace the time-consuming scheme we use now?

Confirming whether the GLUD1 promoter has successfully driven the expression of the target gene or whether the GLUD1 promoter has spatiotemporal specificity is the purpose of our original scheme. On the other hand, post-labeling of GFP (green fluorescent protein) in the target gene can help to screen and obtain stable genetic plants of rice, thus further determining whether our target gene can be specifically expressed in rice seeds and obtain rice lines with high expression of the target product chlorogenic acid, because green fluorescent protein emits light under ultraviolet light, which provides a train of thought.

The suggestions of the two elder students remind us of other possibilities, and these suggestions and ideas provides new thoughts of experimental design.

The forms of corporation with SYSU-CHINA and SJTU-BioX-Shanghai of Sun Yat-sen University and Shanghai Jiao Tong University are introductory lectures on their respective projects. Together, the two teams gave a lecture that included an explanation of the background and objectives of their project, as well as the experimental design.

In order to find a cure for RNA-binding protein (RBPs)-related diseases, SYSU-CHINA and SJTU-BioX-Shanghai wanted to use directed evolution (referring to applying a specific selection pressure to a group that initially had a mutated sequence, forcing that group to evolve in a manner adapted to that pressure) combining the algorithm to find dsRNAs (double strand RNAs) that can inhibit ADAR1 (studies have shown that the absence of ADAR1 increases the sensitivity of tumors to immunotherapy and can improve the efficiency of PD1 therapy).