Team:GDSYZX/Description
DESCRIPTION
COVID-19 & Traditional Chinese Medicine
In 2020, COVID-19 epidemic is raging, affecting the lives of many people. For this public health crisis, our high school students want to do something. In recent years, the clinical practice of atypical pneumonia (SARS) and avian influenza fully suggests that traditional Chinese medicine has a unique role and advantage in antiviral diseases. Compared with the widely used antiviral chemical drugs, natural Chinese herbal medicine has many advantages, including rich resources, low price and small side effects.[1-3] As a starting point, our team tried to use traditional Chinese medicine to achieve our goal.
Honeysuckle
Now commonly used in the treatment of Lianhua Qingwen capsule, Shuanghuanglian granules, there are many honeysuckle as medicinal ingredients. Honeysuckle has a long history of health care and medicine in China. It is a dual-purpose plant for medicine and food, which were known as its remarkable anti-virus, anti-inflammatory and blood pressure lowering effects. The efficacy of honeysuckle has been recorded in ancient books such as Lei Gong's Medicinal Herbs Solution, Materia Medica Fengyuan and Materia Medica Huiyan. [4-5]
Chlorogenic Acid
The main medicinal component of honeysuckle is chlorogenic acid secreted under specific conditions. Chlorogenic acid is a kind of phenylpropanoid synthesized by the condensation of carboxyl group of caffeic acid and hydroxyl group of quinic acid. It is a kind of phenylpropanoid substance synthesized by plant cells through shikimic acid pathway. It has been widely used in the fields of food, medicine and chemical industry. [6-12]
Our Goal
In order to maintain an adequate intake of chlorogenic acid in daily life, our team hopes to achieve high expression of chlorogenic acid in rice. Eating rice is a tradition for most Chinese, and the price is cheaper than honeysuckle. Unfortunately, the expression of chlorogenic acid in rice itself is not enough, so we need to use the gene from honeysuckle to express it more strongly. Honeysuckle has a strong authenticity, some genes must be expressed under specific conditions, so it is not conducive to widespread cultivation and transmission. Our project aims to eliminate this earthliness and let more people enjoy the benefits of traditional Chinese medicine.
What We Did
Our team wants to extract the chlorogenic acid expression gene from the protoplast of honeysuckle and insert it into the gene fragment of rice, so as to solve the problems encountered in the production of chlorogenic acid, so that people can directly ingest chlorogenic acid in the process of daily eating rice, so as to achieve the effect of strengthening health and preventing diseases.
More Information
Reference
[1] Liu Zhonghua, Zhang Wei, Lin Peizheng, etc. The effect of different traditional Chinese medicine compound prescriptions on avian influenza virus infection model mice［J］. Journal of Guangzhou University of Traditional Chinese Medicine, 2010, 27(3): 208-213.
[2] Li Yongping, Li Fuan, Tong Li. Progress in clinical experimental research of antiviral Chinese medicine [J]. Journal of Qinghai Medical College, 2006 (1): 60-67.
[3] Shi Penghui, Qu Xinyan, Zhou Zhe, et al. Research progress of Chinese herbal compound anti-influenza virus [J]. Chinese Journal of Traditional Chinese Medicine, 2017, 32(3): 1172-1175.
[4] Lu Junxian, Liang Ruixue, Lin Huibin. Research progress on anti-influenza virus effects of honeysuckle[J]. Modern Chinese Medicine Research and Practice, 2018, 32(05):81-85.
[5] Zheng Bisheng, Li Huina. Study on the antioxidant activity of the effective components of honeysuckle leaves[J]. Modern Food Science and Technology, 2013, 29(12): 2870-2876.
[6] Lepelley M, Cheminade G, Tremillon N, et al. Chlorogenic acid synthesis in coffee: an analysis of CGA content and real-time RT-PCR expression of HCT, HQT, C3H1, and CCoAOMT1 genes during grain development in C. canephora [J]. Plant Sci, 2007, 172: 978-996.
[7] Duarte GS, Pereira AA, Farah A. Chlorogenic acids and other relevant compounds in Brazilian coffees processed by semi-dry and wet post-harvesting methods [J]. Food Chem, 2010, 118:851-855.
[8] Wang Z, Clifford MN. Comparison of the profiles of chlorogenic acids and their derivatives from three Chinese traditional herbs by LC-MSn [J]. Acta Pharm Sin (药学学报), 2008, 43:185-190.
[9] Naveed M, Hejazi V, Abbas M, et al. Chlorogenic acid (CGA): a pharmacological review and call for further research [J]. Biomed Pharmacother, 2018, 97:67-74.
[10] Bagdas D, Gul Z, Meade JA, et al. Pharmacologic overview of chlorogenic acid and its metabolites in chronic pain and inflammation [J]. Curr Neuropharmacol, 2020,18:216-228.
[11] Li Y, Wang Q, Yao X, et al. Induction of CYP3A4 and MDR1 gene expression by baicalin, baicalein, chlorogenic acid, and ginsenoside Rf through constitutive androstane receptor- and pregnane X receptor-mediated pathways [J]. Eur J Pharmacol, 2010, 640:46-54.
[12] Santana-Gálvez J, Luis CZ, Jacobo-Velázquez DA. Chlorogenic acid: recent advances on its dual role as a food additive and a nutraceutical against metabolic syndrome [J]. Molecules, 2017, 22:358.