Module 2 involves reducing conjugation thereby reducing horizontal gene transfer (HGT). To achieve this, the genes traS and traT are integrated downstream to a medium strength expression system in a F- chassis. The simultaneous expression of these genes in E. coli in a detergent free media has shown to reduce conjugation by 33,000 times (Achtman et al., 1977). The genes traS and traT help in surface exclusion and are carried naturally by the E. coli F factor. These genes have not been exploited in iGEM so far.
This part contains the gene traS downstream to the medium promoter (BBa_J23118) and strong RBS (BBa_B0034), and will be cloned in the vector pSB1C3. Overexpression of traS has been shown to prevent DNA transfer between stable mating pairs.
This part contains the gene traT downstream to the medium promoter (BBa_J23118) and strong RBS (BBa_B0034), and will be cloned in the vector pSB3K3. Overexpression of traT has been shown to prevent the formation of stable mating pairs between bacteria.