Team:Jiangnan China/Part Collection

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The core of our project is to construct a recombinant strain of Starmerella bombicola to produce the sophorolipid step by step and loop by loop. But biosynthesis based on synthetic biology has very high requirements for the basic parts. The chassis microorganism of our project is Starmerella bombicola, which is a chassis microorganism that can naturally produce sophorolipid but is extremely lacking in basic components. Therefore, we obtained 14 Starmerella bombicola endogenous promoters with different gradients through blast, software prediction, and experimental measurement, which not only enriched the basic parts of Starmerella bombicola, but also engaged in more synthesis in the future. It is convenient for researchers who want to use this Starmerella bombicola as a chassis microorganism in synthetic biology.

Part Range: BBa_K3402002-K3402007, BBa_K3402040-K3402047

NO.	Name	Type	Description	Length(bp)
1	BBa_K3402002	Regulator	Ppgi	879
2	BBa_K3402003	Regulator	Ptpi	1481
3	BBa_K3402004	Regulator	Pgki	456
4	BBa_K3402005	Regulator	Peno	1571
5	BBa_K3402006	Regulator	Pgpd	556
6	BBa_K3402007	Regulator	Ptef1	1479
7	BBa_K3402040	Regulator	Ptdh1	1494
8	BBa_K3402041	Regulator	Pfao2	1493
9	BBa_K3402042	Regulator	Ptef2	1478
10	BBa_K3402043	Regulator	Pugta1	1480
11	BBa_K3402044	Regulator	Pmob	1495
12	BBa_K3402045	Regulator	Pugtb1	1483
13	BBa_K3402046	Regulator	Pcyp52m1	1481
14	BBa_K3402047	Regulator	Padh1	1478