Team:Lethbridge HS/Composite Part

Composite Parts

Each of the following basic parts were submitted to the iGEM registry. The parts below are the pectinases used in our project in which all three pectinases are used to improve the breakdown of pectin within organic compost. Each of these enzymes have also been fused to a GFP reporter for purification and assay purposes.

All of our composite parts were designed using the T7 promoter (BBa_I712074), medium strength RBS (BBa_J61100), and double terminator (BBa_B0014).

For an in depth explanation of our construct designs and overall system, please see our Design page. For more construct details please check out our Parts overview pageor click on one of the parts below to be taken to our registry.

Part Name Biobrick Number Construct Composition
Pectin Lyase Pnl BBa_K3349006 T7-mRBS-BBa_K3349000-dT
Pectin Lyase PelB BBa_K3349007 T7-mRBS-BBa_K3349001-dT
Pectin Lyase PelC BBa_K3349008 T7-mRBS-BBa_K3349002-dT
Pectin Lyase Pnl-GFP BBa_K3349009 T7-mRBS-BBa_K3349003-dT
Pectin Lyase PelB-GFP BBa_K3349010 T7-mRBS-BBa_K3349004-dT
Pectin Lyase PelC-GFP BBa_K3349011 T7-mRBS-BBa_K3349005-dT