Team:Lethbridge HS/Composite Part
Each of the following basic parts were submitted to the iGEM registry. The parts below are the pectinases used in our project in which all three pectinases are used to improve the breakdown of pectin within organic compost. Each of these enzymes have also been fused to a GFP reporter for purification and assay purposes.
All of our composite parts were designed using the T7 promoter (BBa_I712074), medium strength RBS (BBa_J61100), and double terminator (BBa_B0014).
For an in depth explanation of our construct designs and overall system, please see our Design page. For more construct details please check out our Parts overview pageor click on one of the parts below to be taken to our registry.
| Part Name | Biobrick Number | Construct Composition |
|---|---|---|
| Pectin Lyase Pnl | BBa_K3349006 | T7-mRBS-BBa_K3349000-dT |
| Pectin Lyase PelB | BBa_K3349007 | T7-mRBS-BBa_K3349001-dT |
| Pectin Lyase PelC | BBa_K3349008 | T7-mRBS-BBa_K3349002-dT |
| Pectin Lyase Pnl-GFP | BBa_K3349009 | T7-mRBS-BBa_K3349003-dT |
| Pectin Lyase PelB-GFP | BBa_K3349010 | T7-mRBS-BBa_K3349004-dT |
| Pectin Lyase PelC-GFP | BBa_K3349011 | T7-mRBS-BBa_K3349005-dT |
