Team:Lethbridge HS/Experiments






Experiments





The following protocols are those we proposed to do in the lab this iGEM season. Feel free to check out our Lab Bookfor more information on what protocols were conducted. We also did some at home composting DIY style; please check out our results page to learn more!



In vitro Colourimetric Assay


At home composting protocol


DNA Purification from Enzymatic Reactions


Phusion PCR


LB Media


DNA Ligation


PCR Product Purification


pJET Cloning


Plasmid Purification


Protein Overexpression


Purification of E. coli his-tagged proteins


Restriction Digest and Dephosphorylation


Bacterial Transformation using Chemically Competent Cells