Team:Lethbridge HS/Experiments


The following protocols are those we proposed to do in the lab this iGEM season. Feel free to check out our Lab Bookfor more information on what protocols were conducted. We also did some at home composting DIY style; please check out our results page to learn more!

In vitro Colourimetric Assay

At home composting protocol

DNA Purification from Enzymatic Reactions

Phusion PCR

LB Media

DNA Ligation

PCR Product Purification

pJET Cloning

Plasmid Purification

Protein Overexpression

Purification of E. coli his-tagged proteins

Restriction Digest and Dephosphorylation

Bacterial Transformation using Chemically Competent Cells