Team:MIT/Parts
Parts
We hope our basic and composite parts are useful for iGEM teams in the future for probing immunopathologies and developing digital, sensitive response systems. Parts were designed as if to be used with the Mammalian MoClo system.
Some of the functionalities of our parts are:
- CRISPR-based endoribonuclease (ERN) developed for use in the PERSIST platform, cleaving at a cut site. Citation: 10.1101/2019.12.15.867150
- The regulatory cutsite that the CRISPR-based endoribonuclease (ERN) cleaves for, developed for use in the PERSIST platform. Citation: 10.1101/2019.12.15.867150
- With binding of the nuclear transcription factor (nTF) to this response element upstream of a minimal constitutive promoter, we can create an "nTF sensitive promoter". Inspired by https://pubs.acs.org/doi/abs/10.1021/acssynbio.5b00266
- With cleavage of this cutsite upstream of endoribonuclease Cas6, we can create an "OFF" switch for Cas6 translation. Inspired by 10.1101/2019.12.15.867150
| Name | Type | Description |
|---|---|---|
| BBa_K3621000 | Coding | CasE (cleaving protein) |
| BBa_K3621001 | Coding | Csy4 (cleaving protein) |
| BBa_K3621002 | Coding | Cas6 (cleaving protein) |
| BBa_K3621003 | Temporary | Cas6 Cutsite |
| BBa_K3621004 | Temporary | Csy4 Cutsite |
| BBa_K3621005 | Temporary | CasE Cutsite |
| BBa_K3621006 | Regulatory | NF-kB responsive element |
| BBa_K3621007 | Regulatory | ELK-1 responsive element |
| BBa_K3621008 | Coding | scFv for IL-1 |
| BBa_K3621009 | Composite | ELK-1 responsive promoter |
| BBa_K3621010 | Composite | NF-kB responsive promoter |
| BBa_K3621011 | Regulatory | CasE cutsite - Cas6 |
| BBa_K3621012 | Regulatory | Csy4 cutsite - Cas6 |
| BBa_K3621013 | Regulatory | Cas6 cutsite - scFv for IL-1 |
| BBa_K3621014 | Coding | ELK-1 Responsive Promoter, CasE |
| BBa_K3621015 | Coding | NF-kB Responsive Promoter, Csy4 |
This page was written by Sangita Vasikaran.