Team:NFLS/Parts
Team NFLS developed the following parts:
New parts
| Part number | part type | Part description | length |
|---|---|---|---|
| BBa_K3513000 | Basic | endoglucanase with signal peptide and linker | 1425 |
| BBa_K3513002 | Composite | endoglucanase with signal peptide, linker and promoter | 1466 |
| BBa_K3513003 | Basic | exoglucanase with signal peptide | 1533 |
| BBa_K3513004 | Composite | exoglucanase with signal peptide, linker and promoter | 1574 |
| BBa_K3513005 | Composite | β-Glucosidase with promoter | 1480 |
| BBa_K3513006 | Basic (part improvement) | JTi1 | 89 |
| BBa_K3513007 | Basic | ChbB | 321 |
| BBa_K3513008 | Basic | ChbC | 1359 |
| BBa_K3513009 | Basic | ChbA | 351 |
| BBa_K3513010 | Basic | CdtC | 570 |
| BBa_K3513011 | Composite | ChbB-ChbC-ChbA | 2043 |
Part improvement
| Origin part | Improved part | Improved function |
|---|---|---|
| BBa_K2976009 | BBa_K3513006 | Improved its function in NF-κB binding |
NF-κB is an important transcription factor. Last year, team 2019_CPU_CHINA built a NF-κB induced promoter. This year, team NFLS improved the efficiency of their part BBa_K2976009 by designing a new part called JTi1 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. This new reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.
We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.
The standard curve:
