Collaboration with AtreXtinct:
Because our team and AtreXtinct have a very similar research direction, and both teams are from China, it is very suitable for us to work together closely. We absorbed countless useful advice from them because they had nearly finished their project at that time.
First of all, AtreXtinct gave us some extremely valuable suggestions about fund-raising. Originally we only purchase some very expensive and fancy objects as the commodities for our street sales such as clocks, statues, and bracelets. According to Zi Qian Wang, the leader of AtreXtinct, nobody was going to buy our products to support our team if our commodities were not very useful. She suggested that we could spend our star-up capital on stationery to target youngsters, who are very interested in high-tech and exquisite stationery. Additionally, AtreXtinct delivered a small lecture to teach us how to write the business plan and showed us their finished work as an example. We gained a deep understanding of the CDSTEP-N Model, SWOT Analysis, and Business Model Canvas. In order to ensure that we were able to make the best out of our time, they helped us to redesign our work schedule and assigned parts of BP to everyone.
Plus, they stressed the importance of working together with the wet lab in an effort to make splendid achievements. We needed to make good communications with the wet lab and spared no effort to coordinate the process. Finally, they also offered us some impressive instructions on propaganda. Since our project was mainly in summer, they thought it would be very beneficial to us to distribute plastic fans to the public which had our team logo on the surface. People would keep them for a very long time and use them frequently in front of the public.
Collaboration with PhosFate:
PhosFate developed a gene-edited E. Coli that can detect the phosphorus concentration in the solution to reduce Phosphate pollution, as the phosphate ion suppresses the expression of the fluorescent GFP. Via comparing the fluorescence of the sample and the standardized data or controlled group, the phosphate concentration of the sample can be estimated. Their method of testing phosphate concentration using their bacteria for estimation of phosphate solubilization ability can be used on Tatumella Citrea.
Preparing 100g modified LB medium containing:
Yeast extract 0.5g
For testing the phosphate solubilizing ability of Tatumella Citrea, three replicate flasks containing LB liquid medium were inoculated with each isolate, using 10 of a bacterial suspension, with 0.5g Ca3(PO4)2 in each flask. Flasks were incubated at 28 for 5 days on an orbital shaker at 100 rpm in the light. Cultures were sampled and autoclaved after 5 days, with 10X dilution with water, for determining the available phosphate concentration via PhosFate’s method.
After sending the sample to Phosphate, we have the results. For the lack of a standardized correspondence chart of the intensity of fluorescent and the phosphate concentration, the specific quantitative results cannot be given.
However, the experience and results indicate the potential of their team’s product as a convenient way of testing phosphate concentration. After the standardized model was established, it can be applied to the isolation process of new phosphate solubilizing bacteria.
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