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Team:PYMS GZ China/Attributions


Attributions

Over the duration of this project, there were many people that contributed to making this experiment successful. We would like to acknowledge those who helped us with this journey. Spanning the globe, our students from China and the United States diligently worked for months in order to produce all the data, results, and project components. Along the way, we have had outside contacts that provided us invaluable guidance throughout the project. We would like to take a moment and recognize those who made this experience possible.

Dry Lab Team

Charlotte Zhang was in charge of creating the content for the website. She oversaw the Dry Lab Team, created the methodologies for our team’s Dry Lab work, and helped put the wiki together. She designed the project and assembled the all-girls team. She coordinated communication between the US and China members and oversaw progress. Also, she created the coloring book.

Oulan Li was the primary coder for our website. Additionally, she created the explanatory diagrams, made graphics, and designed the wiki. She is one of the initiators of this project.

Taylor Shimizu was in charge of creating the content for the website. She oversaw the Dry Lab Team, designed the science communications methodologies, and helped put the wiki together. Also, she finalized project design and proposed implementation.

Emily Roshan was in charge of creating the content for the website. She oversaw the Dry Lab Team, created the methodologies for our team’s Dry Lab work, and helped put the wiki together. Also, she was responsible for video production and human practices.

Alanna Xue was responsible for organizing and executing the public engagement. She was primarily in charge of creating our Boss Women in STEM podcast, Benefit Concert, and interviewing researchers to help guide our design.

Christy Douglas was tasked with executing our human practice methodology. She was a key contributor to creating our Elementary School Synthetic Biology Game and conducted interviews with experts in the field of virology that provided our team with important information.

Nicole Douglas was in charge of continuing our human practices. She interviewed diversity experts in order to create our Latinx Outreach Strategy. She was also a graphic designer for our website.

Hannah Yang made images for the Synthetic Biology Coloring Book and the Elementary School Synthetic Biology Game. Also, she completed Dry Lab methodology.

Wet Lab team

Leyi Zheng was the overall leader and coordinator of the Wet Lab team. She was in charge of wet lab experiment design and execution. She was primarily responsible for constructing plasmid, point mutation, cell culture, transfection, and antibody neutralization and data analyzing.

Xulinzi Cai was part of the Wet Lab team, and focused on the cloning, gel extraction purification, fluorescent detection, and cell culture.

Guanghui Sun was part of the Wet Lab team, and focused on the point mutation, cell culture, and pseudovirus infection and neutralization. She was also in charge of two interviews with doctors.

Jieying Gan was part of the Wet Lab Team, and focused on gel extraction purification, fluorescent detection, and cell culture. She was also in charge of two interviews with doctors.

OuqiaoLi was part of the Wet Lab team, and focused on the gel extraction purification, cloning, and fluorescent detection. She was also in charge of coordinating with the reagents company.

Our Instructors

Our Primary PI - Ms. Qiyu Li
As the key person of our project, she gave us a lot of professional advice and taught us a set of courses on Biosafety and Ethics. She shared external resources, such as finding lab space and seeking professionals for assistance. She also organized team works between team members and ensured the team was following the safety rules throughout the process.

Special thanks to:

Professor Jie Zhu
As a director of the lab in Medical University, she provided the facilities and lab space for our team. By frequent discussions with us, she provided knowledge and advice on bioethics, safe lab practice, and guided us through difficulties encountered during experiments, for example, modification on the method to generate the S-D614G variant.

Varun Govil: He served as our guide and mentor during the project. As our team worked tirelessly to conduct all the experiments and create all the content for the project, Varun helped steer our work to make it as meaningful and impactful as possible. We couldn’t have done this project without him!

Professor Qi Zhang
As a director of a lab in Sun Yat-Sen University, she helped us to interview some important experts and arranged the team to visit the State Key Laboratory of Respiratory Disease in Guangzhou, China.

Dr. Gen Li
As a research fellow, he supervised our team lab experiments and taught us techniques as well as aiding us in protocol design. He gave us advice on project design and experiment planning, helped troubleshooting experiments.

Dr. Zhongcheng Zhou
Research Fellow, he assisted us in designing our DNA constructs as well as offering advice on lab techniques and helped us to analyze the data and troubleshoot experiments.

Dr. Jingyun Yang
As a first author of Nature paper and an experienced researcher (A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity, Nature, 2020), he provided some important plasmid, antibody and serum for us.

Dr. Jingyun Yan
Dr. Yan gifted us important materials that let us execute our project. With the donation of HEK293 ACE2 cells and monkey serum, we were able to create our general assay system and test the neutralization of the antibodies, simulating a vaccine.

Olivia Lam: She contributed multiple graphics to our website.

Zixing Zou: Zixing was involved in coding the website.

Race to Cure Foundation: Provided funding for the competition.