Notebook
Part 1: Experimental Records
Part 2: Notable Advances
Notable Advances
On July 2nd, we finished constructing pet28a-modification.
On July 21st,we did our lyophilization experiment for the first time.
On July 29th, we finished constructing pet28a-sfGFP.
On August 10th, we finished constructing pet28a-modification-106094 and pet28a-modification-107838.
On August 18th, we got the first positive result for our lyophilization experiment.
On August 24th, we finished constructing pet28a-modification-sfGFP and pet28a-modification-33020-106094.
On August 27th, we finished constructing pet28a-modification-J100/J107/J109-sfGFP.
On September 17th,we confirmed that the survival rate could improve with the increase of 106094’s expression.
On September 23rd,we confirmed that pet28a-modification-33020-106094 could improve the resistance of bacteria under lyophilization environment.
On September 25th,we finished constructing pet28a-modification-J100-106094-pdtA.
Experimental records
The record of molecular cloning
The record of freeze-drying, fluorescence detection and SDS-PAGE