Team:SJTU-BioX-Shanghai/Protocol
Protocol
Contents
1.Agarose Gel Preparation
2.Amp Antibiotics Mother Solution Preparation
3.Chemoreceptor Cells Preparation
4.Cm Antibiotics Mother Solution Preparation
5.Electrotransformation(Electric transfer)
6.Enzyme Digestion
7.Enzyme Linkage
8.Error Prone PCR
9.Flow Analyzer
10.Gel Extraction and Purification
11.Gibson Assembly
12.IPTG induce expression of pET28a vector protein
13.KOD neo Plus PCR
14.Mix PCR
15.Plasmid Extraction
16.Select Monoclonal Colony
17.Solid liquid LB Preparat
18.Sterilization
19.TBE
Index 1-5
1.Agarose Gel Preparation
2.Amp Antibiotics Mother Solution Preparation
3.Chemoreceptor Cells Preparation
4.Cm Antibiotics Mother Solution Preparation
5.Electrotransformation(Electric transfer)
Index 6-10
6.Enzyme Digestion
7.Enzyme Linkage
8.Error Prone PCR
9.Flow Analyzer
10.Gel Extraction and Purification
Index 11-15
11.Gibson Assembly
12.IPTG induce expression of pET28a vector protein
13.KOD neo Plus PCR
14.Mix PCR
15.Plasmid Extraction
Index 16-19
16.Select Monoclonal Colony
17.Solid liquid LB Preparat
18.Sterilization
19.TBE
SJTU-BioX-Shanghai
contact us
Contact us: sjtuigem@gmail.com or sjtuigem@gmail.com
Bio-X Institute, Shanghai Jiao Tong University, Dongchuan Rd. 800
Copyrights © 2020 SJTU-BioX-Shanghai
