HZAU-China
6.22th
We found many similarities between our projects with the HZAU-China team during a casual chat at CCiC's QQ Communication Group.Our project is designed in the testing section for Streptococcus mutans and uses the same two-component system that is owned by Streptococcus mutans.We thus embarked on the first step of our cooperation.We started talking about each other's projects that was the first step in our long-term cooperation.
7.6th
It was the first time that we started the online meeting with HZAU-China.We described the details on the project design and ideas at that time,exchanged each other's projects,and discussed how to model.
7.16th
In this daily communication,we would like to conduct a questionnaire survey on the current dental caries and their population.When designing the questionnaire,the members of the HZAU team guided us through the questionnaire together.From this questionnaire,we know that only 9 percent of the hundreds of people regularly go to dental clinics for check-ups.This makes us start thinking whether the rest of the population thinks regular testing is too cumbersome or that tooth decay doesn't matter.
Questionnaires published with HZAU-China
7.29th
In this daily meeting,we raise the question of the recent experiment: the suicide system has not been working and we hope HZAU-China team will give us some new ideas. HZAU-China team suggested that if the experimental steps were correct and there were still problems,we could consider checking the sequence we designed.
8.6th
That day we suddenly realized that it was difficult to come up with more new ideas about each other's projects and synthetic biology. So we have an idea to have a team project meeting together.We invited seven iGEM teams to share their projects and welcomed all interested parties to our conference so that more people could get interested in synthetic biology.
8.11th
During the period leading up to the event,we actively organized the participating teams and contacted the authorities to release the meeting information,and the HZAU-China team was also actively designing conference posters and invitations from the teams.
8.15th
At the same time,we found many similarities between the NWU-CHINA-A antimicrobial peptide project team at this meeting.Later,we also launched cooperation in this field!
Post a screenshot of the message for Friend Team Meetup on the IGEM Event page
8.16th
We held a small seminar after the previous day's exchange to summarize the results of the project.While sorting through the meeting,we found that the chassis microorganisms originally used in the HZAU team project would produce substances that were harmful to tooth enamel,so we recommended that the HZAU team replace the chassis with a safer and more viable E.coli Nissle 1917.
9.19th
We had a weekly discussion that day as usual, and I found that both sides had problems with WIKI's code editing. We pinned the file to the DW when the code was uploaded, but there was a rendering problem at the time of the upload, and the HZAU team suggested that we could place the code file directly on the mediawiki. We found that in the HZAU team's code editing,the style of the wiki overrides their original style and has not solved this problem,and we recommend that the HZAU team find and reset the styles on the wiki.
9.30th
Throughout the season,we and the HZAU team have been close partners in supporting each other and helping each other. So we suggested to HZAU-China that we might be able to hold a workshop on synthetic biology together to promote synthetic biology to more people.Therefore,on this day,we invited HZAU-China to work with the SZPT-CHINA team to promote synthetic biology to our university students and to spread a lot of knowledge about tooth decay.
Screenshot of lecture
10.15th
For special reasons this year, many teams were unable to return to the lab early to conduct experiments. The HZAU team only entered the lab in September this year, and in the early project exchanges the HZAU team and we all used some of the same biological components, such as nlmc starters. To help the HZAU team better complete the project, we shared the same part of the experiment in the hope of helping the HZAU team.
NWU-CHINA-A
6.14th
At the South China Exchange Conference was held by SZU-China,the NWU-CHINA-A team had a preliminary understanding of our project.At the same time,we were also concerned that their project was similar to ours in terms of sterilization.
Project introduction of NWU-CHINA-A
7.6th
We found NWU-China-A based on the team contact information at the South China Exchange Meeting.Due to time constraints,the leader of our two teams had the first exchange.During the one-hour communication,NUW-CHINA-A pointed out that perhaps braces are not suitable as the final product of the project,and suggested that we choose more convenient and fast products,such as toothpaste,mouthwash and other portable products.
7.25th
This day the NWU-CHINA-A team held a Shan an xi exchange meeting.Through the report of the NWU-China-A team that day,we believe that the projects of two sides do have similarities.At the same time, we are very interested in the broad-spectrum antimicrobial peptides in their projects,because we did not find highly selective antimicrobial peptides in the sterilization section so we hope to find better results of broad-spectrum antimicrobial peptides.
Shananxi iGEM Meetup
8.15th
In this exchange meeting between us and the HZAU-China team,we and NWU-CHINA-A reported their respective projects in detail.
After our project report ended,NWU-CHINA-A raised questions about our products.They believed that if fluorescent protein is used as the final visualization,users will not be able to easily observe whether they have dental caries.
At the same time,NWU-CHINA-A recommends that if we want to make the product more convenient and concise,maybe we can replace the final fluorescence with enzymes visible to the naked eye.We think the NWU-CHINA-A proposal is very constructive,so in September,we immediately replaced the fluorescent protein in the detection part with the visible hydrolase lac-z.
Friendship Meet up
9.5th
Online communication
At this weekly meeting, we exchanged the current progress of the experiment on both sides and asked each other's current problems.We are not sure which antimicrobial peptide is better for the treatment part.NWU-China-A proposed that a broad-spectrum antimicrobial peptide was used in their project,and was willing to give us the amino acid sequence to suggest that we can test the effect with other candidate antimicrobial peptides.
Give DCD1-L broad-spectrum antimicrobial peptide (His-tag)
Communication with NWU-CHINA-A
9.13th
Also at this daily regular meeting,we discussed the current situation of our respective projects and evaluated the projects of both parties as a whole.Before that,a suicide switch was added to the project.After learning about this idea, we suggested that NWU-CHINA-A might be able to use our switch based on glucose concentration last year.
Protein purification protocol
9.20th
Our project has stalled on protein purification, so we hope to get help from our partners So we opened a small discussion meeting.After communication, we found that the NWU-CHINA-A team seemed to be facing the same problem as us.So we thought about whether we could exchange purified protein solutions and try to give each other some advice. We suggest that the NUW-China-A team may be able to purify proteins using ultrafiltration centrifuges.
Mascot with NWU-CHINA-A
10.4th
Judging from the lectures organized by us and the HZAU-China team, the feedback from college students is generally good. Many students are still discussing the hazards of dental caries after our lecture, as well as new treatment methods (using synthetic biology methods)
Based on the results of the previous session,we decided to hold a seminar with NWU-CHINA-A once again to promote synthetic biology,and the theme was determined as: antibiotics and synthetic biology.
Because we have set a joint lecture on popular science antimicrobial peptides,and for this we also designed the mascots of SZPT-CHINA and NWU-China-A to facilitate the promotion of later lectures.
Screenshot of lecture