We highly appreciated all the groups and individuals that contributed to our project, we won't achieve such a success without your strong support. We are sincerely grateful!
TEAM
Mingxuan Chi：Team leader.
Besides being in charge of everything, he was the designer of ssDNA reporter and RPA efficiency test. He produced high-quality crRNA by in vitro synthesis.
Yijing Cai: Wet lab member.
She is good at protein extraction and purification. She mainly dealt with Cas12a and the characterization of Cas12a.
Zehua Yin: Wet lab member.
He mainly carried the improvement of Cas12a, including introducing point mutations and designing experiment to test the effect. He also participated in making videos.
Mengning Luo: Wet lab member.
She mainly carried the improvement of Cas12a, including introducing point mutations and designing experiment to test the effect. She also helped find experts in Human Practices.
Zhiyu Fang: Bioinformatics specialist.
As the only member majoring in bioinformatics, he used various software tools to calculate the protospacers for Cas12a to recognize. He also participated in modelling and RPA testing.
Qinyi Chu: Wet lab member.
She participated in constructing plasmids. She was also responsible for our collaboration with NJU-China.
Ruijie Xiang: Wet lab member.
He constructed most of the plasmids and he was the designer of our aptamer-sensing module. He also participated in Cas12a characterization by western-blotting.
Yujie Wen: Chief video editor.
He did a great job in making our promotion video and presentation video as a very amateur editor. As the Giant Jamboree was unexpectedly changed to online form, he stood up and bravely took the responsibilities of making videos. He also participated in RPA testing and education.
Sizhe Gu: Wiki constructor.
Majoring in computer science, he is better in deal with codes than any others in the team. With the career plan of studying deep learning, he also participated in constructing our neuronal network model.
Ruiyan Xia: Human practices specialist.
She leaded all of our Human practices activities. and she also participated in constructing our wiki pages.
Wenxin Li: Wet lab member.
She participated in the improvement of Cas12a and she also played important roles in our Human Practices activities.
Zefang Zhao: Wet lab member.
She participated in constructing plasmids and protein purification.
Zeyang Zhang: Fund manager.
He was in charge of our team fund. He also participated in the improvement of Cas12a.
Rui Su: Modelling member.
He constructed our ring-dropping model and gave a very accurate prediction of the volume of liquid we should use in our final product.
Haochen Lv: Art designer.
He made our poster and all the art elements in our project. He also participated in the purification of RNA.
Yuxin Song: Art designer.
She made our poster and all the art elements in our project.
Advisors
Kaida Zhang:
Provided tutoring about experiments.
Provided advices on presentation and wiki.
Helped in holding HP activities.
Helped in fund management.
Yi Liu:
Provided tutoring about experiments.
Provided advices on expanding the potential of our device.
Provided advices on presentation and wiki.
Helped in our collaboration with ZJUT_China_B.
Principal Investigators
Dr. Haitao Yang:
Provided advices on safety about dealing with antibiotic resistance.
Provided advices on presentation and wiki.
Dr. Wei Shen:
Provided advices on experiments.
Provided advices on presentation and wiki.
Special Thanks
Dr. Xingxu Huang & Dr. Xinjie Wang,
School of Life Science and Technology, ShanghaiTech University
Advised on design of ssDNA.
Dr. Quanjiang Ji,
School of Physical Science and Technology, ShanghaiTech University
Pointed that antibiotics abuse was an important question which should be paid attention to.
Advised on the tools used for testing the antibiotic resistance.
Advised on crRNA synthesis procedure.
Adopted biological safety.
Dr. Chao Zhong,
MSBC, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Provided instruments for system characterization, ELIASA
Dr. Wei Li,
Qilu Hospital of Shandong University
Advised on improvement of our design.
Pointed our equipment should be high-throughput.
Introduced common and important antibiotic resistance genes.
Dr. Zujun Sun,
Tongji Hospital of Tongji University
Advised on improvement of our design.
Pointed our equipment should be mobile and quantitive.
Dr. Zhiying Zhang,
Tongji Hospital of Tongji University
Introduced methods of testing antibiotic resistance used for patients for treatment.
Pointed the equipment we designed should be cheaper and more rapid to achieve the result.
Mr. Yungang Li,
Shandong Provincial Center for Animal Disease Control and Prevention
Introduced methods of testing antibiotic residues used for animals and the inconvenience of the current methods.
Pointed the key point of our design should be cost-efficient.
Advised on the size of our equipment.
Mr. Fei,
Liyuan Farm
Showed us the possible usage scenario of our product.
Introduced the situation of antibiotics used in farm.
Dr. Liping Wu,
The 2nd Affiliated Hospital Of Chengdu Medical College
Introduced the inconvenience of methods used for testing antibiotic resisitance.
Yunjie Xiao,
Tianjing University
Provided plasmid that contains AsCpf1 gene.
Sponsorship
ShanghaiTech University
Provided almost all of our fundings, facilities and venues.
Provided our students other supports of carrying out our project.
SnapGene
Provided us their software without charging, which greatly promoted our progress.
