Plasmids contruction
TOPO cloning:
Hieff Clone® Zero TOPO-Blunt Cloning Kit -Hieff Clone® Zero TOPO-Blunt Cloning Kit.pdf
PCR:
PrimeSTAR® Max DNA Polymerase -PrimeSTAR-Max-DNA-Polymerase—fast-and-high-fidelity-PCR.pdf
Tsingke Golden Mix -Tsingke Golden Mix.pdf
CWBIO 2×Es Taq MasterMix (Dye) -2×Es Taq MasterMix（Dye）.pdf
DNA purification
DNA clean up:
TIANquick Midi Purification Kit -https://en.tiangen.com/content/details_69_5465.html
DNA gel purification:
TIANgel Purification Kit -TIANgel Purification Kit-200903.pdf
Plasmid extraction:
TIANprep Rapid Mini Plasmid Kit -DP105 -TIANprep Rapid Mini Plasmid Kit-180103.pdf
RNA preparation
T7 in vitro transcription:
HiScribe™ T7 High Yield RNA Synthesis Kit -https://international.neb.com/products/e2040-hiscribe-t7-high-yield-rna-synthesis-kit#Protocols,%20Manuals%20&%20Usage
RNA purification:
crRNA preparation -crRNA preparation.pdf
Cas12a preparation
Cas12a extraction and purification -Cas12a extraction and purification.pdf
Protein SDS-PAGE electrophoresis protocol -Protein SDS-PAGE electrophoresis protocol.pdf
