Team:ShanghaiTech China/Experiments

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Description

Protocols
 
 
 
 

Plasmids contruction

TOPO cloning:

Hieff Clone® Zero TOPO-Blunt Cloning Kit -Hieff Clone® Zero TOPO-Blunt Cloning Kit.pdf

PCR:

PrimeSTAR® Max DNA Polymerase -PrimeSTAR-Max-DNA-Polymerase—fast-and-high-fidelity-PCR.pdf

Tsingke Golden Mix -Tsingke Golden Mix.pdf

CWBIO 2×Es Taq MasterMix (Dye) -2×Es Taq MasterMix(Dye).pdf

DNA purification

DNA clean up:

TIANquick Midi Purification Kit -https://en.tiangen.com/content/details_69_5465.html

DNA gel purification:

TIANgel Purification Kit -TIANgel Purification Kit-200903.pdf

Plasmid extraction:

TIANprep Rapid Mini Plasmid Kit -DP105 -TIANprep Rapid Mini Plasmid Kit-180103.pdf

RNA preparation

T7 in vitro transcription:

HiScribe™ T7 High Yield RNA Synthesis Kit -https://international.neb.com/products/e2040-hiscribe-t7-high-yield-rna-synthesis-kit#Protocols,%20Manuals%20&%20Usage

RNA purification:

crRNA preparation -crRNA preparation.pdf

Cas12a preparation

 
 

Cas12a extraction and purification -Cas12a extraction and purification.pdf

Protein SDS-PAGE electrophoresis protocol -Protein SDS-PAGE electrophoresis protocol.pdf

Aptamer related materials

 
 

Aptamer-sensing protocol -Aptamer-sensing protocol.pdf