Favorite basic part
Compared to the existing part BBa_I732005 which is a full-length lacZ, we insert hERα and intein into the lacZ to produce a new part BBa_K3525000, giving it a new function of detecting the presence of estrogenic chemicals in environmental water.
In order to confirm the color reaction of lacZ reporter protein, we tested and confirmed the color reaction of ONPG and lacZ protein.
Figure 1: Color reaction result of ER sensor protein with ONPG in the presence of estrogen
1: Negative control, 2: color reaction of ER sensor protein expression bacteria lysate without estrogen, 3: color reaction of ER sensor protein expression bacteria lysate with estrogen.
We found that the bright yellow color reaction of ONPG and lacZ is more suitable for visual inspection than the blue color reaction of x-gal. We confirmed that the optimum reaction temperature of ONPG and lacZ was 37 ℃.
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