We obtained a chimeric protein composed of S. cerevisiae VMA intein, the esterogen binding domain of hERα and lacZ reporter protein. This protein may provide a complete and independent biosensor for estrogen molecular. In theory, estrogen dependent self splicing and production of active lacZ protein can be realized by this chimeric protein. The related domain parts and design principles may also be used for sensor proteins of other molecules.
Composite part BBa_K3525001 contains ER-sensor-S protein coding sequence. It is used to express and purify ER-sensor-S protein .
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