Professor Yang gave us an online presentation about the project.
All of us gathered in Shanghai, had ice-breaking activities, designed our logo, did self-introduction, and selected team leader candidates. We were divided into two different laboratories to do experiment.
we learned safety precautions and got familiar with experimental equipment. Then did PCR to clone genes and made LB medium.
We did PCR again to clone genes 1, 2, 3, 4, and 5. We also did gel recycling to purify these productions.
Several overlap PCR were done to get 1+2, 3+4, 1234+5
We constructed plasmid successfully and sequenced in a company.
The sequencing result was compared and analyzed. And the plasmid was transformed into E. coli competent cell DH5αand BL21(DE3).
We expressed ER sensor protein.
We purified the LacZ protein and SDS-PAGE gel electrophoresis was done to check the protein we obtained.
we also collaborated with Listeria monocytogenes team.
We did functional test.
We also took our team photos.
Analyze experimental data, summarize and answer questions.
We did a live presentation on zoom software then finished our training.
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