Team:TJUSLS China/Safety

<!DOCTYPE html> Team members

Introduction
Prior to Experiment
Safe Labwork
References

Introduction


The degradation of PET with high crystallization has attracted worldwide attention. PET is one of the important factors causing white pollution, which has caused great harm to the environment. Our work will yield unlimited benefit to the environment, rather than do harms.

We promise none of the organisms we use pose a risk to the safety and health of team members, and no organism can cause infection or adverse health effects or symptoms. 

The detailed safety measures can be found below.

Prior to Experiment


When coming up with our project, we regard the safety assessment as a necessity. After all we have to ensure team members' health before aiming at theraupetics.

First and foremost, we completed the safety form and used the check-in forms as a precaution to obtain permission of working with these antibiotic resistance genes from iGEM Safety and Security Committee.

Safety form and Check-in


We regard the safety assessment as a necessity. So we completed the safety form on time and used the check-in forms as a precaution to obtain permission of working.

Biosafety Training


We have been given a course called Biosafety and bioethics. The teachers informed us of all the potential risks and dangers associated with the use of different chemicals, techniques, and equipment, as well as how we could minimize these risks.

This year, our team won't do any lab work due to COVID-19. We promise that in next year we would continue our lab work following these aspects

Safe Labwork

Personal Safety


Kind of bacteria


Our project poses little risk to the students, as for the only organism used in our project is a commonly used lab-strain of Escherichia coli, which is designated as a biosafety level 1 organism.


Facilities


Aseptic techniques are always used when handling live culture or confexting some buffer.

Super clean bench is used when doing any operations about living bacteria.

Gloves and lab coats are required to wear as soon as we step into the laboratory. Our dressing seriously follow BSL-1 laboratory standard.

Emergency eye washer is situated outside of our lab, together with aid supplement and fire extinghuishers.


Environmental Protection


The only organism used in our project is a commonly used lab-strain of Escherichia coli, which is designated as a biosafety level 1 organism.

All of the operations related to living bacteria carried with them are in the clean bench. All of the bacterial liquid is collected specially in a waste bucket with high concentration of NaOH to kill phage and change the structure of DNA. Used tubes and plates are all autoclaved.

Subsequent to Experiment


Waste disposal


All of the waste would be divided into relevant categories and collect each of them separately. There are some mini dustbins on the table collecting pipettes and microtube, and by the end of a day's experiment we will pull them out into dust bin in the lab. And the person on cleaning duty that day is responsible for changing the yellow garbage bag and taking the full bag to school's collection site.

Useless bacterial liquid are pulled in a bucket together with high concentration NaOH. This bucket is experimented with two lids, meeting our country's standard.


Hygiene


Our lab will have different person on cleaning duty everyday in turn and all of our team members are on the list. Not only should this one deal with garbage, but he is also required to wipe and mop the ground, clean the public table, check facilites and note all of his tasks in the chart.

70% ethanol is used for disinfection anytime.

Everyone has formed the habit of washing hands with antibacterial hand sanitizer at the exit of labtorary when finishing experiments.

Reference


[1] iGEM team TJUSLS_China2019. Safety. Oct., 2019.

https://2019.igem.org/Team:TJUSLS_China/Safety

[2] Measures for the Administration of Biosafety and Environment in Pathogenic Microbiological Laboratories. State Environmental Protection Administration.2006.03.08

http://www.mee.gov.cn/gkml/zj/jl/200910/t20091022_171837.htm

[3] Regulations on Biosafety Management of Pathogenic Microorganism Laboratories. China State Council.2010.01.14.

http://www.mee.gov.cn/home/ztbd/swdyx/2010sdn/zcfg/201001/t20100114_184290.shtml



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